pHANNIBAL植物干扰质粒

基本信息

启动子:	35S promoter
原核抗性:	氨苄青霉素Amp
克隆菌株:	大肠杆菌Stbl3
5'测序引物:	35S: CTATCCTTCGCAAGACCCTTC

质粒简介

               pHANNIBAL是一个植物细胞RNAI干扰质粒

质粒图谱

质粒序列

LOCUS       Exported                5826 bp ds-DNA     circular SYN 03-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(19..35)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(215..643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        670..774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             775..1635
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1806..2394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2865..4208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          4236..5004
                     /gene="pdk"
                     /number="3"
                     /label=PDK intron
                     /note="pyruvate orthophosphate dikinase intron from 
                     Flaveria trinervia"
     terminator      5051..5757
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(5798..5816)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
       61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
      181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
      241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
      421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
      481 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
      541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
      601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
      661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc
     2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc
     2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat
     2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga
     3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt
     3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga
     3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc
     3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag
     3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct
     3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac
     3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag
     3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat
     3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa
     3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca
     3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc
     3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg
     3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
     3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
     3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
     3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
     3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
     4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
     4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     4201 agaggacacg ctcgaggaat tcggtacccc aattggtaag gaaataatta ttttcttttt
     4261 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt
     4321 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa
     4381 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat
     4441 ttttaatgaa tttgatcgaa catgtaagat gatatactag cattaatatt tgttttaatc
     4501 ataatagtaa ttctagctgg tttgatgaat taaatatcaa tgataaaata ctatagtaaa
     4561 aataagaata aataaattaa aataatattt ttttatgatt aatagtttat tatataatta
     4621 aatatctata ccattactaa atattttagt ttaaaagtta ataaatattt tgttagaaat
     4681 tccaatctgc ttgtaattta tcaataaaca aaatattaaa taacaagcta aagtaacaaa
     4741 taatatcaaa ctaatagaaa cagtaatcta atgtaacaaa acataatcta atgctaatat
     4801 aacaaagcgc aagatctatc aattttatat agtattattt ttcaatcaac attcttatta
     4861 atttctaaat aatacttgta gttttattaa cttctaaatg gattgactat taattaaatg
     4921 aattagtcga acatgaataa acaaggtaac atgatagatc atgtcattgt gttatcattg
     4981 atcttacatt tggattgatt acagttggga agctgggttc gaaatcgata agcttggatc
     5041 ctctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
     5101 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
     5161 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
     5221 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
     5281 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
     5341 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
     5401 aagactgatt acataaatct tattcaaatt tcaaaaggcc ccaggggcta gtatctacga
     5461 cacaccgagc ggcgaactaa taacgttcac tgaagggaac tccggttccc cgccggcgcg
     5521 catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa gttacgggca
     5581 ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg agaattatgc
     5641 agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg
     5701 acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac
     5761 ctgcaggcat gcaagctagc ttactagtga tgcatattct atagtgtcac ctaaatctgc
     5821 ggccgc