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提供定制载体
血清型 | 组织嗜性 |
---|---|
AAV1 | 平滑肌、中枢神经系统、肺、视网膜、胰腺、心脏、肝脏 |
AAV2 | 平滑肌、中枢神经系统、肝脏、肾脏、视网膜 |
AAV3 | 平滑肌、肝脏、肺 |
AAV4 | 中枢神经系统、视网膜、肺、肾 |
AAV5 | 平滑肌、中枢神经系统、肺、视网膜 |
AAV6 | 平滑肌、心脏、肺、脂肪、肝脏 |
AAV6.2 | 肺、肝 |
AAV7 | 平滑肌、视网膜、中枢神经系统、肝脏 |
AAV8 | 平滑肌、中枢神经系统、视网膜、肝脏、胰腺、心脏、肾脏、脂肪 |
AAV9 | 平滑肌、肺、肝、心脏、胰腺、中枢神经系统、视网膜、睾丸、肾脏 |
AAVrh10 | 平滑肌、肺、肝、心脏、胰腺、中枢神经系统、视网膜、肾脏 |
AAV-DJ | 肝、心、肾、脾 |
AAV-DJ/8 | 肝脏、大脑 |
AAV-P-HP.eB | 中枢神经系统 |
AAV-P-HP。S | PNS |
AAV2-复古 | 脊神经 |
AAV2-四头肌 | 内皮细胞 |
5' ITR: 5' inverted terminal repeat. In wild type virus, 5' ITR and 3' ITR are essentially identical in sequence. They reside on two ends of the viral genome pointing in opposite directions, where they serve as the origin of viral genome replication.
Promoter: The promoter that drives your gene of interest is placed here.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
SV40 late pA: Simian virus 40 late polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
3' ITR-Δtrs: AAV 3' ITR with a deleted terminal resolution site. The presence of the mutated 3’ITR leads to the generation of single-stranded, inverted repeat genomes with a mutated ITR in the middle and a wild type ITR at each end. This facilitates intramolecular base pairing within the mutant ITR extending through the genome resulting in the folding of the viral DNA to form a double-stranded molecule.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.
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引用 | 主题 |
---|---|
专家Rev Hematol。4:539 (2011) | scAAV载体在基因治疗中的进展和挑战 |
莫尔瑟。16:1648 (2008) | scAAV载体的研究进展与应用综述 |
吉恩·瑟。10:2112 (2003) | 通过突变AAV终端重复生成scAAV载体 |
莫尔瑟。16:1648 (2008) | 自互补的AAV载体;进步和应用。 |