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- Enzo Life Sciences
- USA
- BML-HW8600-0001
- 2025年07月13日
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- 供应商：
Neobioscience
- 保存条件：
-80
- 规格：
1 x 1.0 x mg
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文献和实验See “Notes” below on making stock sucrose solution. Procedure Note: Nucleoli were prepared from HeLa cells using a variation on a method described by Muramatsu and co-workers in 1963 (Muramatsu M, Smetana, K., and Busch, H.: Quantitative aspects
A. SONICATION 1. Prepare the starting material of 2.5x109 HeLa nuclei. Suitable HeLa nuclei can be purchased from 4C Biotech (Seneffe, Belgium; Cat no: CC-01-30-25). Add S1 solution to the thawed nuclei to a final volume of 30ml. Mix thoroughly but avoid
Isolate It All：siRNA • miRNA • Total RNA • Native Protein
from the same number of HeLa cells in triplicate. Analysis of 1 µg RNA on a denaturing agarose or acrylamide gel showed that 28S, 18S and 5.8S rRNA were quantitatively recovered in the fraction depleted in small RNA. In contrast, RNAs smaller than 200 nt, such as 5
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