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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
Fraction II (HeLa)
- 库存：
大量
- 供应商：
欣博盛
- 规格：
1*1mg
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文献和实验Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
CHAPS Insoluble Floating Fraction Preparation
7.5, 50 mM NaCl, 2 mM EDTA). They were finally resuspended in TEBP (TEB plus various protease inhibitors: 1x PIC I and 1x PIC II) to 2 x 108 /ml. CHAPS powder was added at 20 mg/ml. After gentle mixing, the lysed cells were kept on ice for 3-5
In Vitro Transcription of Class II Promoters in Higher Plants
Class II gene in vitro transcription systems from animal cells (1 –3 ) or fungi (4 ) have been essential for elucidating the mechanisms regulating gene expression. Crude protein extracts from Hela cell nuclei that allow accurate
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