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    CRL-7601 Hs 865.Sk 人皮肤成纤维细胞

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    CRL-7601 Hs 865.Sk 人皮肤成纤维细胞

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    • 货号: Hs 865.Sk
      数量: 大量
      ATCC Number: CRL-7601
      ATCC® Number: CRL-7601™ Price: $429.00
      Designations: Hs 865.Sk
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: adherent
      Organism: Homo sapiens (human)
      Morphology: fibroblast
       
      Source: Organ: skin
      Disease: normal
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Age: 59 years
      Gender: female
      Ethnicity: Caucasian
      Comments: The line was established from apparently normal tissue from a patient who had adenocarcinoma of the stomach.
      Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
      Please see the NBL Repository description.
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Temperature: 37.0°C
      Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
      Medium Renewal: Every 2 to 3 days
      Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
      Add fresh culture medium, aspirate and dispense into new culture flasks.
      Preservation: culture medium 95%; DMSO, 5%
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
      recommended serum:ATCC 30-2020

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