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NCI-H128 人肺小细胞肺癌细胞

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  • NCI-H128
  • 2025年12月15日
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    上海复祥生物科技有限公司
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    • 供应商：

      复祥生物

    • 运输方式：

      常温或干冰

    • 生长状态：

      正常

    • 库存：

      大量

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    Designations: NCI-H128
    Depositors: AF Gazdar
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: floating aggregates
    Organism: Homo sapiens
    Morphology: floating aggregates
    Source: Organ: lung
    Disease: carcinoma; small cell lung cancer
    Derived from metastatic site: pleural effusion
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Tumorigenic: Yes
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 10
    D13S317: 12
    D16S539: 13
    D5S818: 9
    D7S820: 8,10
    THO1: 6,9
    TPOX: 8
    vWA: 17
    Cytogenetic Analysis: There are 2 distinct aneuploid peaks, both have characteristic 3p deletion.
    Isoenzymes: AK-1, 1
    ES-D, 1
    G6PD, A
    GLO-I, 1-2
    Me-2, 1
    PGM1, 1
    PGM3, 1
    Age: 60 years
    Gender: male
    Ethnicity: Black
    Comments: This cell line is aneuploid.
    Will form colonies in soft agar.
    It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.
    NCI-H128 cells do well on a rotary shaker flask at 70 to 80 rpm at 37C.
    It is normal for cultures of this line to have fairly large amounts of cell debris.
    Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20% - OR - Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
    Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: Twice per week
    Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days.
    Preservation: Culture medium, 90%; DMSO, 10%
    Related Products: normal (or near-normal) cell line established from the same patient:ATCC CRL-5947
    References: 1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201
    23036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156
    23037: . . Cancer Res. 40: 4556-4563, 1980.
    23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
    23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258
    23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370
    32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

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