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Herpes simplex virus Vesicular stomatitis virus Human poliovirus 1
Tumorigenic:
No
Reverse Transcript:
negative
Cytogenetic Analysis:
modal number = 78; range = 62 to 109. The stemline number is hypotetraploid. Most of the cells contained only telocentric or acrocentric chromosomes.
Age:
embryo; 14 to 17 day gestation
Comments:
The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos [PubMed: 4301006].The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus [PubMed: 4306523]. Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition