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- 规格:
100 µg
蛋白名称:Galectin 8/LGALS8蛋白, Galectin 8/LGALS8 protein
蛋白构建:A DNA sequence encoding human LGALS8 (AAF19370.1) (Met 1-Trp 317) was fused with the GST tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 76 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.2-1 μg/ml.
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human LGALS8/GST chimera consists of 550 amino acids and predicts a molecular mass of 62.7 kDa. The apparent molecular mass of rh LGALS8/GST is approximately 60 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:AAF19370.1
蛋白氨基酸序列:Met1-Trp317
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验kinases activate the serine/threonine kinases M n k l and M n k 2 . EMBO J. 16, 1909-1920. 13. Fujita, H., Fujita, H., Takemura, M., et al. (2003) A n ArabidopsisM A D S - b o x protein, A G L 2 4 , is specifically bound to and phosphorylated
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
蛋白质定量分析(Protein determination)
1. 每槽先加好50 μL标准品 (#0, #1 … #5) 或未知样本 (X1, X2),以二重复加入96 孔滴定盘中,如图所示。 微量滴定盘的使用 2. 每槽再加入200 μL Dye-Reagent (d),在全部样本槽加完前,不要中断添加;同时小心避免气泡产生。 3. 轻拍滴定盘一侧,使添加的各成份均匀混合,注意Dye-Reagent 密度较大,很容易沉积在下方而分层
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