小鼠 Src蛋白|Src protein|Src(Mouse, N-GST & His)(产品说明)
蛋白名称:Src蛋白, Src protein
蛋白构建:A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. The specific activity was determined to be >80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001020566.111
蛋白氨基酸序列:Met1-Leu535
蛋白标签:N-GST & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.