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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse IL2RA / CD25 Protein (ECD, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25190.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:CD25/IL2RA蛋白, CD25/IL2RA protein
蛋白构建:A DNA sequence encoding the mouse IL2RA (NP_032393.3) extracellular domain (Met 1-Lys 236) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 22
蛋白分子量:The secreted recombinant mouse IL2RA cmprises 226 amino acids with a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of the rm IL2RA is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_032393.3
蛋白氨基酸序列:Met1-Lys236
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Chikaishi W, et al. Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer.Thoracic cancer, PubMed ID: 37674354
after specifically interacting with its receptors. Human IL-2 is a 133-amino acid polypeptide with a molecular mass of 15-18 kDa. IL-2 signaling is mediated by a multichain receptor complex consisting of an alpha (CD25), beta (CD122), and gamma (CD132) chain. The IL-2R
after specifically interacting with its receptors. Human IL-2 is a 133-amino acid polypeptide with a molecular mass of 15-18 kDa. IL-2 signaling is mediated by a multichain receptor complex consisting of an alpha (CD25), beta (CD122), and gamma (CD132) chain. The IL-2R
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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