小鼠 gp130/IL6ST蛋白|gp130/IL6ST protein|gp130/IL6ST(Mouse, C-His)(产品说明)
蛋白名称:gp130/IL6ST蛋白, gp130/IL6ST protein
蛋白构建:A DNA sequence encoding the mouse gp130 (NP_034690.3) extracellular domain (Met 1-Glu 617) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to bind mouse IL11Ra in a functional ELISA. 2. Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 19
蛋白分子量:The secreted recombinant mouse gp130 consists of 606 amino acids and has a predicted molecular mass of 68 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_034690.3
蛋白氨基酸序列:Met1-Glu617
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.