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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human Coagulation Factor XIII B chain / F13B Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:Human Coagulation Factor XIII B chain / F13B Protein (His Tag)
蛋白构建:A DNA sequence encoding the human F13B (P05160) (Met 1-Thr 661) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 21
蛋白分子量:The secreted recombinant human F13B consists of 652 amino acids and predictes a molecular mass of 74.5 kDa. In SDS-PAGE under non-reduced conditions, the apparent molecular mass of rh F13B is approximately 65 kDa.
蛋白NP号:P05160
蛋白氨基酸序列:Met1-Thr661
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验system that originates from membrane shuttling proteins such as the Drosophila homeobox protein Antennapedia, the HIV-1 transcriptional factor TAT and VP22 from HSV-1 has advantages for targeted delivery compared with standard translocation techniques. This transport
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
A, B and C neurotoxinI453Clostridium botulinum neurotoxin (BoNT) serotype A and BI, 78 mg/L[57]Clostridium botulinum neurotoxin, serotype AI, 450 mg/kg cells308Clostridium botulinum neurotoxin heavy chain fragment, serotype BI, 390 mg/g[58]Clostridium
机会,减少蛋白质的聚集;b、去污剂及表面活性剂:如Trition X-100、CHAPs、磷脂、磺基甜菜碱等对蛋白质复性有促进作用,但它们能与蛋白质结合,很难去除;c、氧化-还原剂:对于含有二硫键的蛋白,复性过程中应加入氧化还原体系,如GSH/GSSG、DTT/GSSG、DTE/GSSG等,氧化还原系统通过促进不正确形成的二硫键快速交换反应,提高了正确配对的二硫键的产率[19];d、小分子的添加剂:如盐酸胍或尿素、烷基脲、碳酸酰胺类等,都可阻止蛋白聚集,它们的作用可能为:稳定蛋白的活性状态、降低非正确
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