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Heterologous Proteins Expressed in Pichia pastoris

有以下几部分组成：

Protein Comments
Mode, amount,
signal sequence
References and Links

分别分物种来源如下：
Bacteria

Alpha galactosidase
S
453

Bacillus licheniformis alpha-amylase
S, 2.5 g/L, SUC2
[53, 54]

Bacillus stearothermophilus D-alanine carboxypeptidase
S, 100 mg/L, native
[55]

Bacillus thuringiensis cry 2 gene product
S, α-MF
455

Bordetella pertussis Pertussis pertactin (P69)
I, 3 g/L
[56]

Metarhizium anisopliae chymotrypsin S, α-MF, active
322

Chlostridium botulinum serotypes A, B and C neurotoxin
I
453

Clostridium botulinum neurotoxin (BoNT) serotype A and B
I, 78 mg/L
[57]

Clostridium botulinum neurotoxin, serotype A
I, 450 mg/kg cells
308

Clostridium botulinum neurotoxin heavy chain fragment, serotype B
I, 390 mg/g
[58]

Clostridium botulinum neurotoxin serotype F
I, 240 mg/kg cells
307

Clostridium botulinum neurotoxin serotype A binding domain
I, 2.4 mg total
[59]

Clostridium tetani Tetanus toxin fragment C
I, 12 g/L
[60]

Diphtheria anti-human anti-T cell immunotoxin
S, 10 mg/L, α-MF or Kluyveromyces lactis killer toxin signal, functional
454

Escherichia coli acid phosphatase/phytase (appA2)
S, 28.9 U/mg
[61]

Escherichia coli β-Galactosidase
I, 2.0 ´ 103 U/mg
[62]

Escherichia coli β-D-glucuronidase
I
309

Escherichia coli β-Lactamase
I
[63]

Leishmania major cathepsin B-like protease
S, α-MF
[64]

Providencia rettgeri penicillin G amidase
S, 0.18 U/ml, active
452

Staphylococcus aureus staphylokinase
S, 50 mg/L, α-MF
[65]

Streptococcus equisimili streptokinase
I, 77 mg/L,

S, 3000 IU/ml, active
66, 319]

Streptomyces subtilisin inhibitor
S
[67]

Streptomyces clavuligerus deacetoxycephalosporin C synthetase
S, 120 mg/L, α-MF,

active
451

Streptomyces viridosporus T7A endoglucanase
S, 2.47 g/L total protein, α-MF
[68]

Streptomyces viridosporus T7A peroxidase
S, 2.47 g/L total protein, α-MF
[68]

Toxoplasma gondii SAG1 antigen
S, 12 mg/L, α-MF
[69]

Thermus aquaticus YT-1 aqualysin I
S, 1 g/L, native signal sequence or α-MF (100mg/L)
456

Vibrio cholerae accessory cholera enterotoxin (Ace)
S, 7 mg/L, α-MF
[70]

Fungi

Alternaria alternata Alt a 1 allergen
S, α-MF
[71]

Alternaria alternata rAlt a 2 allergen
S, 180mg/L, α-MF, functional
326

Aspergillus awamori glucoamylase
S, 400 mg/L, native
72

Aspergillus awamori glucoamylase catalytic domain
S, 400 mg/L, PHO1
[73]

Aspergillus fumigatus Asp f 2 allergen
I, weak reactivity
323

Aspergillus fumigatus catalase L
S, 2.3 g/L, PHO1
[74]

Aspergillus fumigatus dipeptidyl peptidase IV (DPP IV)
S, PHO1
[75]

Aspergillus fumigatus dipeptidyl peptidase V (DPP V)
S, 0.15 mg/L, PHO1
[76]

Aspergillus fumigatus phytase
S, 730 mg/L, α-MF, active
320

Aspergillus giganteus a-sarcin ribotoxin
S, 1 mg/L, synthetic native,
PHO1
[77]

Aspergillus niger β-glucosidase
S, 0.5 g/L, PHO1
313

Aspergillus niger phytase (phyA)
S, 65 U/ml, α-MF
[78]

Aspergillus niger endo-β-1,4-xylanase
S, 60 mg/L, SUC2, native
311

Barley glucosidase
345

Candida albicans MNT1 mannosyltransferase S, 250 mg/L, α-MF, active
324

Candida antarctica Lipase B S, α-MF, 25 mg/L, active
321 457, 559

Candida guilliermondii xylose reductase gene (xylI)
I, 0.65 U/mg;
S, 0.18 U/mg, α-MF
[79]

Candida rugosa lipase 1 (CRL)
S, 150 U/ml, α-MF
[80]

Candida rugosa LIP2 lipase
S, 2.3 mg/L, α-MF, active
463

Candida rugosa lip4 lipase
S, 100mg/L, α-MF, active
459

Fusarium solani pectate lyase (pelC)
S, 1 mg/L, PHO1
[81]

Fusarium solani pectate lyase (pelD)
S, native
[82]

Geotrichum candidum lipase isoenzymes
S, 60 mg/L, α-MF
[83]

Kurtzmanomyces sp. 1-11 lipase
S, 46U/ml, α-MF, active
464

Metarhizium anisopliae chymotrypsin
S, α-MF, active
322

Mucor pusillus rennin
S, active
310

Neurospora crassa NOP-1
I, 2.2 mg/L, PHO1, active
312

Neocallimastix patriciarum cellulase 6A-Candida Antarctica lipase B fusion protein
S, 1.5 g/L, active
466, 559

Phanerochaete chrysosporium glyoxal oxidase
S, 2g/L, α-MF, active
325

Phanerochaete chrysosporium cellobiose dehydrogenase
S, 72 mg/L, α-MF, active
461

Phanerochaete chrysosporium β-glucosidase
S, 28,500 U/L, active
465

Phaeseolus vulgaris aspartic protease
See ref 45 in331


Phytophthora cryptogea β-cyptogein
S, 45 mg/L, PHO1
[84]

Pleurotus sajor-caju laccase isozyme S, α-MF, active
462

Pycnoporus cinnabarinus I-937 laccase S, 8 mg/L, α-MF and endogenous, active
318

Rhynchosporium secalis avirulence gene product NIP1 I, 16 mg/L, active
350

Rhizopus oryzae (also other yeast and bacteria)lipase
S, 60 mg/L, α-MF, active(200U/ml)
[85, 389

Saccharomyces cerevisiae CUP1 promoter and Pichia genes
Copper-inducible 316

Saccharomyces cerevisiae Hsp70
I, 21mg/g cells, active
467

Saccharomyces cerevisiae invertase
S, 2.5 g/L, native
[86]

Saccharomyces cerevisiaeKex 2p
S, 100 mg/L, active
458

Saccharomyces cerevisiae Ktr1p
S, 400 mg/L, PHO1
[87]

Saccharomyces cerevisiae (α-1,2-mannosyltransferase)
S, 40 mg/L, PHO1
[87]

Schizophyllum commune vitamin B2-aldehyde-forming enzyme
S, 120 mg/L, α-MF
[88]

Trametes versicolor (white rot fungus) laccase (lccI)
S, 1.6 g/L, native and α-MF
[89], 314, 560, 561

Trichoderma harzianum β-(1-6)-glucanase
S, 9.3 mg/L
[90]

Trichoderma reesei cellobiohydrolyase I
S, α-MF, low activity
315

Trichoderma reesei cellobiohydrolyase Cel7A
active
327

Trichoderma reesei 1,2-α-kD-mannosidase
S, 17.5 mg/L, α-MF, active
317

Protists

Actinia equina(sea anemone)equistatin
S, 1.7 g/L, α-MF, active
424, 471

Aquorea victoria green fluorescent protein
S, 5 mg/L, α-MF, active
472, 476

Chondrus crispus red alga hexose oxidase
I (secretion signals failed), some activity
[91], 469

Gracilariopsis lemaneiformis red alga a-1,4-glucan lyase (GLq1)
I
[92]

Mougeotia scalaris recombinant phytochrome
I, 100 mg/L, active
470

Plasmodium falciparum merozoite surface protein 1 (MSP-1)
S, 24 mg/L, α-MF
[93]

Plasmodium falciparum antigen Pfs48/45
I (1% total protein) or S, α-MF, inactive
448

Plasmodium falciparum antigen P30P2MSP119
S, 500 mg/L, α-MF, effective
468

Plasmodium falciparum subtilisin-like protease pfsub1
I, 0.5g/L
449

Plasmodium vivax apical membrane antigen I (AMA-1)
S, 50 mg/L, PHO1
[94]

Ryschosporium secalis avirulence gene product NIP1
I, 16 mg/L, PHO1, active
334

Reticulomyxa filosa (giant freshwater amoeba) a2, b2 tubulin isoforms
I, 400 mg/g
[95]

Trypanosoma cruzi acid α-mannosidase
S, 11.5 mg/L, native
[96]

Plants

Allium sativum (garlic) alliin lyase
I, 2.167 U/g
[97]

Apple 1-aminocyclo-propane-1-carboxylate(ACC) synthase
I, 10 mg/L, active
333

Arabidopsis thaliana NADH:nitrate reductase
I, 18 mg/g
[98,99]

Arabidopsis thaliana aldehyde oxidase
I, active
337

Arabidopsis thaliana AtJ2 cochaperone protein
250 mg/L,
350

Arabidopsisthaliana xylosyl transferase
S, 25ug/L
490

Barley (Hordeum vulgare) sucrose fructan 6-fructosyl transferase
S, α-MF
[100]

Barley a-amylase 1
S, 50 mg/L, native
[101]

Barley a-amylase 2
S, 1 mg/L, native
[101]

Barley aleurone tissue a-glucosidase
S, α-MF
[102]

Brassica napus memb.-anchored endo-1,4-β-glucanase
S, α-MF, active
484

Cassava(Manihot esculenta) P-450
I, 30 nmol/L, active
328

Cellulomas fimi family 2A carbohydrate-binding module
S, α-MF, active
330

Coffee bean a-galactosidase
S, 400 mg/L, α-MF, active
[103, 340, 341]

Corn(Zea maize) nitrate reductase
I, 42 mg/L, active
342

Corn(Zea maize) cytokinin oxidase
S, native
[111]

Cowpea (Vigna unguiculata) aspartic protease
See ref 45 in 331

Cynara cardunculus (cardoon) cyprosin
S, 1 mg/L, native
[104]

Cynodon dactylon (Bermuda grass) Cyn d 1
S, 1.5 g/L, PHO1
[105,106]

Galanthus nivalis agglutinin
S, PHA-E, α-MF, 2 mg/L
[107, 346]

Ginsing small medicinal peptice
S, 800 mg/L, α-MF, active
492

Grass group 1 allergins(β-expansins)
S, active
335

Hevea brasiliensis hydroxynitrile lyase
I, 22 g/L
[108]

Hevea brasiliensis Hev b 7 patatin-like allergen
S, 10 mg/L, α-MF
[109,110]

Horseradish peroxidase S, active
344

Ipomoea batatas L. invertase S, 4 mg/L, α-MF, active
488

Mustard trypsin inhibitor 2 S, 160 mg/L, α-MF
347

Oat phytochrome A, phA
I, 30 mg/g
[112,113]

Oat phytochrome A, phyA65 apoprotein
I, 20 mg/g
[114]

Olea europaea (olive tree) aeroallergen Ole e 1
S, 60 mg/L, α-MF
[115, 334, 391]

Olea europaea 1,3-β-glucanase Ole e 9 C-term.seg.
S, 100 mg/L, α-MF, active
486

Oleaceae sp. (lilac and privet) pollen allergens
S, 30 mg/L, reactive
481

Pepper endo-b-1,4-glucanase cCel1
S, α-MF
[116]

Pepper endo-b-1,4-glucanase cCel2
S, native
[116]

Persea americana (avocado) prs a 1 major allergen
S, 50 mg/L, α-MF
[117]

Phaseolus vulgaris agglutinin(PHA)
S, 1 mg/L and 100 mg/L, active
[346], 493

Pisum sativum antifungal protein Psd1
S, 13.8 mg/L, α-MF, active
483

Pisum sativum copper amine oxidase
S, 1.4 mg/L, active
338

Pisum sativum mitochondrial pyruvate dehydrogenase
I, active
343

Plantago lanceolata pollen allergen Pla l 1
S, 20 mg/L, active
489

Pokeweed antiviral protein
S, 12 mg/L, α-MF, active
348

Potato phytochrome B
I, 25 mg/g
[118]

Potato proteinase inhibitor II
S, α-MF, active
329

Ragweed allergen Amb a 6
S, 1 mg/L, α-MF
[119]

Rice α-amylase
S, α-MF
339

Soybean root nodule acid phosphatase
S, 10 mg/L, α-MF
[120]

Spinach glycolate oxidase
I, 250 U/g
[121, 122]

Spinach nitrate reductase
I, 420 mg/L, active
342

Spinach phosphoribulokinase
I, 0.5 mg/g
[123]

Solanum tuberosum ATP-diphosphohydrolase
S, 1 mg/L, active
487

Sugar beet defensin, AX2
S, active
336

Talaromyces stipitatus feruloyl esterase
S, 300 mg/L, active
491

Timothy grass group I allergen
S, α-MF
[124]

Tomato prosystemin
I, very low yield
332

Tomato Lycopesicon esculentum Mill. LeMir (L. esculentum miraculin)
S, PHO1
[125]

Wheat lipid transfer protein
S, 720 mg/L, PHO1
[126]

Wheat puroindoline-a protein
S and Mb-bound, 14 mg/L
482

Invertebrates

Achacina fulica Ferussac (giant African snail) achacin
S, 0.2 mg/L, native
[127]

Acyclostoma caninum (hookworm)
S, active
388

Aplysia californica (marine mollusk) ADP ribosyl cyclase
S, 300 mg/L, α-MF
[128, 361

Aqueora victoria (jellyfish) green fluorescent protein
I, S, PHA-E
[129]

Arabidopsis thaliana α-1,3-fucosyltransferase homologues
S, α-MF, active
485

Bombyx mori lysozyme
S, 1.1 mg/L, α-MF and native signal, active
562

Boophilus microplus (cattle tick) Bm 85
I, 2 g/L, effective
353

Boophilus microplus (cattle tick) Bm86
I, undefined, 1.5 g/L, SUC2
[131, 132, 133]

Cockroach allergen, Bla g 4
S, 50 mg/L
[134]

Dermatophagoides pteronyssinus Der p 1
S, 150 mg/L, α-MF, active
563

Diabrotica virgifera virgifera(corn rootworm) cysteine proteinase
S, α-MF, active
357

Drosophila melanogaster double-stranded specific adenosine deaminase
I, active
362

Drosophila melanogaster tRNA-specific adenosine deaminase
I, 133 ug/L
356

Drosophila melanogaster angiotensin I-converting enzyme
S, 160 mg/L, α-MF
[135]

Drosophila melanogaster core α-1,3-fucosyl-transferase
S, low activity
490

Drosophila melanogaster malonyl-CoA-sensitive carnitine palmitoyltransferase
I, active
355

Drosophila melanogaster GFP-actin fusion
I,active
478

Firefly luciferase
I (peroxisome)
[136]

GAVACÔ vaccine against cattle tick
S, 2.0 g/L
[137]

Haementeria ghilanii (South American leech) ghilanten
S, 10 mg/L, α-MF
[138]

Hirudo medicinalis (leech) hirudin
S, 1.5 g/L, α-MF
[139]

Honeybee odorant-binding protein (ASP2)
S, 150 mg/L, native
[140]

Honeybee olfactory protein
S, 0.2g/L, native
[305]

Antarctic krill euphauserase
S, 500 μg/L, α-MF, active
358

Leech hirudin-albumin fusion protein
S, 15 mg/L, α-MF, active
475

Lumbricus rubellus serine protease
S, α-MF, active
474

Nematode anticoagulant protein c2
Active
367

Nematode elastase inhibitors
S, active
390

Nippostrongylus brasiliensis (parasitic nematode) non-neuronal secreted acetylcholinesterase
S, 50mg/L, α-MF, active
[141, 354]

Olea europaea Ole e 9 C-teminal

Panulirus argus (spiny lobster) cytochrome P-450
I, 20 pmol/mg microsomal prot., active
351

Pseudaletia separata (armyworm) growth-blocking peptide
S, α-MF, 50 mg/L, active
477

Shistosoma mansoni asparaginyl endopeptidase
S, α-MF, active
352

Snake venom α-bungarotoxin
S, 2 mg/L, α-MF
359

Spider dragline silk protein
I, 663 mg/L
[142]

Tick anticoagulant peptide
S, 1.7 g/L, PHO secretion sig.,active
[143

Yellowjacket and paper wasp venom allergen A5 S, 100 mg/L, α-MF
360

Vertebrates (non-human)

Bovine β-casein
I, 1 g/L
[150]

Bovine enterokinase catalytic domain
S, 6.3 mg/L, α-MF
[144]

Bovine follicle stimulating hormone β-subunit
S, 4 mg/ml, α-MF
[145]

Bovine β1,4-galactosyltransferase I
S, 11mU/L, α-MF, active

Bovine β-lactoglobulin variants
S, α-MF, 100 mg/L
497

Bovine IFN-omega 1
S, 4 mg/L, SUC2
[146]

Bovine interferon-stimulated gene product 17
S, 6 mg/L, α-MF, active
380

Bovine β-lactoglobulin
S, >1 g/L, α-MF
[151,152,153, 387]

Bovine lysozyme c2
S, 550 mg/L, native
[147]

Bovine mannose 6-phosphate receptors
S, α-MF, 28 mg purify. protein/L
517

Bovine opsin
undefined, 0.3 mg/L, PHO1
[148]

Bovine pancreatic trypsin inhibitor (aprotinin)
S, 930 mg/L, α-MF
[149]

Bovine tissue-type plasminogen activator (tPA)
S, 1.1 mg/L, α-MF
[154]

Bovine transcobalamin
S, α-MF
[306]

Brushtail possum TNFα
S, α-MF
[155]

Bungarus fasciatus (snake) venom gland acetylcholinesterase
S, 2 mg/L, native
[156]

Cat Fel d 1 and albumin
S, 3.3 mg/L, PHO1
393

Chicken mutant egg white lysozyme
S, α-MF, 2.1 mg/L, active
512

Chicken liver a-N-acetylgalactosaminidase
S, 11.6 mg/L, α-MF, PHO1
[157]

Chicken α-tropomyosin
I, 20 mg/L, active
496

Electrophorus electricus acetylcholinesterase AchE type T
S, native
[158]

Equine lactoferrin
S, α-MF, 40 mg/L, active
502

Feline type I interferons
S, α-MF, active
518

Goat α-lactalbumin
S, α-MF, 20 mg/L
506

Hen lysozyme
S, 20 mg/L, α-MF
[159]

Herring antifreeze protein
S, α-MF, active
377

Mammalian amyloid precursor fusion protein
S, α-MF, active
378

Mammalian lipocalin allergen Bos d2
S, mg amounts, native
[160]

Mammalian α2,6-sialyltransferase
I and S, active
401

Mouse ABC transporters
I, active
513

Mouse activin type II receptor
S, 5 mg/L, active
395

Mouse single chain antibody Fv fragments (sFv)
S, 250 mg/L, α-MF, PHO1
[169, 365, 366, 369, 371, 372

Mouse Fv heterodimer
S, 4 mg/L, α-MF, active
564

Mouse CC Chemokine
S, α-MF, active
394

Murine endostatin
S, 700 mg/L, α-MF
170, 510, 515

Mouse epidermal growth factor
S, 450 mg/L, α-MF
[162]

Mouse gelatinase B
S, 10 mg/L, α-MF
[163]

Mouse P-glycoprotein
I, 700ug/100mg memb. protein, active
376

Mouse Mdr3 P-glycoprotein
I (membrane-bound), 6 mg/mg
[166,167,168, 383]

Mouse monoclonal single-chain Fv antibody fragment
S, α-MF, 45 mg/L, active
498, 509

Murine macrophage inflammatory protein-2 (MIP-2)
S, 40 mg/L, α-MF
[172]

Mouse lysosomal acid a-mannosidase
S, native
[164]

Murine Golgi mannosidase IA
S, PHO1
[171]

Mouse P-glycoprotein mdr3
I, active
570

Mouse Apha-methylacyl-CoA racemase
I, active
375

Mouse 5HT5A 5-tryptamine receptor
undefined, 40 pmol/mg,

α-MF
[161]

Mouse salivary α-amylase
S, 240 mg/L ,active


Mouse sonic hedgehog protein
S, α-MF, 500 mg/L, active
392

Mouse major urinary protein complex (MUP)
S, 270 mg/L, native
[165]

Recombinant non-hydroxlated gelatins(based on rat and mouse collagen)
S, 14.8 g/L, α-MF
385

Ostrich egg-white lysozyme
S, α-MF, 10 mg/L, active
511

Ovine follicle stimulating hormone (oFSH)
S, 22 mg/L, α-MF
[173]

Ovine interferon-τ
S, 392mg/L, active
516

Pig liver esterase
S, α-MF, 500U/mg protein, active
494

Porcine carboxypeptidase B
S 250 mg/L, native, active
384

Porcine follicle-stimulating hormone
S, 10 mg/L, PHO1
[174]

Porcine inhibitor of carbonic anhydrase (transferrin family)
S, 5 mg/L, α-MF
[175]

Porcine single-chain interleukin-12
S, 4 mg/L,α-MF, active
370

Porcine leukocyte 12-lipoxygenase
I
[176]

Porcine lactoferrin
S, 12 mg/L, α-MF or endogenous signal
507

Porcine pepsinogen
S, PHO, 30 mg/L, active
504

Rabbit intestinal peptide transporter (PepT1)
I
[177]

Rabbit intestinal peptide transporter (PEPT2)
I
[178]

Rabbit antibody: monoclonal single-chain Fv specific for recombinant human leukemia inhibitory factor
S, 100 mg/L, α-MF
[179]

Rabbit plasma cholesteryl ester transfer protein
S, PHO1
[180]

Rabbit testicular angiotensin converting enzyme
S, PHO1, native
[181]

Rabbit tissue factor (thromboplastin)
I, 92 mg/g wet cell paste, active
519

Rat acetycholinesterase
S, 1 mg/L, native
[156]

Rat brain acetylcholinesterase T subunit
S, 100 U/L α-MF
[182]

Rat calretinin gene fragments
S, 200 mg/L, α-MF, functional
379

Rat carnitine palmitoyltransferase I and II
I, active
373, 386

Rat CD59
S, 100 mg/L, α-MF
381

Rat complement regulator, crry
S, α-MF
[183 , 381]

Rat Golgi sialoglycoprotein MG160
S, 10 mg/L, α-MF
[184]

Rat high-mobility group 1 (HMG 1)
S, 50 mg/L, α-MF
[185]

Rat intrinsic factor
S, 40 mg/L, α-MF, functional
447

Rat liver mitochondrial carnitine palmitoyl transferases I and II (CPTI and II)
I (mitochondria)
, 187]

Rat multidrug resistance protein MRP6
I, active
570

Rat NO synthase reductase domain
I, 25 mg/L
[188]

Rat odorant-binding protein
S , 80 mg/L, α-MF, same as wild type
364

Rat peroxisomal multifunctional enzyme (perMFE-II)
I
[189]

Rat procathepsin B
S, 100 mg/L, α-MF
[190, 191]

Rat serotonin transporter
I, inactivr
514

Rat tumor necrosis factor-α
I, 10 mg/L, active
382

Sea raven type II antifreeze protein (SRAFP)
S, 36 g/L, α-MF, stable
[192, 193], 499

Shark 17 a-hydroxylase/C17,20-lyase
I
[194]

Syrian golden hamster prion protein PrPc
I,<0.1 mg/L
[195]

Tricosurus vulpecula (possum) cytokine stem cell factor (SCF)
S, α-MF, 45 mg/L
501

Humans

a(1,3/4) fucosyltransferase
S, 30 mg/L, α-MF
[130]

a-1,2-mannosidase 1B w/o TM domain
S, α-MF
[196]

a-N-Acetylgalactosaminidase (a-NAGAL)
S, 11.6 mg/L, α-MF
[197]

Antibody: scFv (against ovarian carcinoma)-biotin mimetic peptide
S
[263, 264]

Antibody: scFv (against squamous carcinoma)
S, 50 mg/L, α-MF
[245]

Antibody: scFv (against prostate-specific antigen)
S, α-MF, 20 mg/L, functional
524

Antibody: single-chain Fv-Fc fusions
S, 4 mg/L, active
525

Antibody: catalytic scFv
S, α-MF, 4 mg/L, active

Antibody: Fab (against atrazine)
S, α-MF, 40 mg/L, active
530

Antithrombin
active

α1-antitrypsin (α1-AT)
S, inulinase signal sequence
[198]

β2-adrenergic receptor
undefined, 25 nmol/g, α-MF
[161]

ADAR1, ADAR2,ds-RNA-specific adenosine deaminases
I, 1 mg/L
[200]

Alzheimer’s disease amyloid precursor protein α, β, and γ-secretase products
S, PHO1
[201]

Alzheimer’s disease amyloid precursor protein, 2 domains
S, 24 mg/L, 0.1 mg/L, α-MF
[202]

Amyloid precursor-like protein 2 (APLP2)
S, 40 mg/L, α-MF
[203]

Amyloid precursor protein (APP)
S, 24 mg/L, PHO1
[204, 205]

Amyloid precursor proteins, rAPP695, rAPP770
S, 4.5 + 1 mg/L, native
[206]

Antithrombin III
S, S. cerevisiae SUC2, 320 IU/L, active
531

BP230 antigen
I, 480 ug/L
546

Bile salt stimulated lipase
S, 1 g/L, native, INV
[207], 521

Bivalent diabody against carcinoembryonic antigen (CEA), T-cell coreceptor CD3-cell coreceptor CD2
S, 1 mg/L, α-MF
[208]

c-kit receptor kinase domain
I, 0.2 mg/L
[209, 210]

Carcinoembryonic antigen
S, 20 mg/L, α-MF
[211, 406

Heart muscle carnitine palmitoyltransferase I (M-CPTI) and II (CPTII)
I (mitochondria), active
232, 430,

386, 186

Caspase-3
I, 1 mg/g
[212]

Cathepsin K
S, 38 mg/L, α-MF
[213, 214]

Cathepsin L propeptide
S, 10 mg/L, α-MF
[215, 216]

Cathepsin V
S, α-MF
[217]

Cathepsin X
S, 5 mg/L, α-MF, activable
417

CD14
S, 20 mg/L,α-MF, active
414, 550

CD38
S, 455 mg/L, α-MF
[218]

CD40 ligand soluble form
S, 255 mg/L
[219]

CD140, soluble
S, α-MF, functional
522

Chimeric B7-2 antibody fusion protein
S, 15 mg/L, α-MF
[220]

Chitinase
S, 400 mg/L, α-MF, active
533

Chorionic gonadotropin α subunit, β subunit, and αβheterodimer
S, 24 mg/L (a), 3mg/L (b),
16 mg/L (ab), α-MF, active
[221, 428]

chymase
S, 200ug/L, SUC2, active
418

Chymotrypsinogen B
S, 480mg/L, authentic
526, 527

Fibroblast collagenase (proMMP-1)
S, 2.3 mg/L, α-MF
[229]

Type I collagen
I, 0.5 g/L, active
528, 540

Type III collagen (with prolyl 4-hydroxylase)
I, 15 mg/L
[288], 540

C1r complement serine proteinase
S, 1.5 mg/L, ,α-MF, active
434

C1 inhibitor
S, α-MF, 180 mg/L, active
541

Complement receptor type 1
S, 50 mg/L, α-MF, binds C3b
407

Cromer blood group antigen decay-accelerating factor
S, α-MF
[222]

Cytomegalovirus ppUL44 antigen
I, 0.1 mg/ml
[223]

Decay accelerating factor-DAF (CD55)-Echovirus-7-receptor
S, 6 mg/L, α-MF
[224]

Dipeptidyl peptidase IV (DP IV)
S, 0.5mg/L, active
551

Double-stranded RNA-specific editase I (hREDI)
I, 1 mg/L
[225]

Elastase
S, α-MF, active
403

Endostatin
S, 20-200 mg/L, α-MF
[170, 226, 397

Erythropoietin receptor variant
S, 200 mg/L
439

Fas ligand
S, 100 mg/L, α-MF or PHO1, active
[227, 431]

Fibrinogen, 143-411, 143-427
S, 100 mg/L, 75 mg/L, α-MF
[228]

Fibrinogen-420 aEC domain
S, α-MF
[230]

Α-1,3-fucosyltransferase VI
S, α-MF, active(3U/L)
415

β-1,4-galactosyltransferase
S, α-MF, active(0.6U/L)
415

Galβ1,3-glucoronosyltransferase(GlcAT-1)
I or S, α-MF,active
421

Gastric cathepsin E
S, 0.6 mg/L, native
[231]

Gelatinase B
S, 50 mg/L, α-MF, active
425

Glucocerbrosidease
S, α-MF
538

Glutaminyl cyclase
S, α-MF, active
535

N-glycosylation enzymes
S, I , active
549

Gp17/GCDFP-15
S, 3 mg/L, α-MF, active
399

Gp130
S, α-MF, active
416

Granzyme B
S, 1 mg/L, α-MF, active
432

Granulocyte colony stimulating factor
S, α-MF, 10 mg/ml, active
523

Growth factor-β type II receptor
S, 20 mg/L, α-MF, active
545

11β-hydroxysteroid dehydrogenase 1/carbonyl reductase
I, active
396

11β-HSD 1B variant
I, active
548.0

Human serum albumin
S, 13.4mg/g biomass
536

Insulin
S, synthetic signal or α-MF, 1.5 g/L, active
[233], 532

Insulin-like growth factor-1 (IGF-1)
S, 600 mg/L, α-MF
[234

Interferon α-1
S, α-MF, 35 mg/L, active
520

Interferon gamma receptor cytoplasmic domain
I
[235]

Interleukin-17 (hIL-17)
S, 0.35 mg/L, α-MF
[236]

Intracellular proteinase inhibitor (PI-6)
I, 50 mg/L
[237]

Intrinsic factor
S, 40 mg/L, α-MF, functional
447

Kunitz-type protease inhibitor domain of protease nexin-2/Amyloid b-protein precursor
S, 1.0 g/L, α-MF
[238]

Leukaemia inhibitory factor (LIF)
S, 17 mg/L, α-MF
[239]

Lewis fucosyl transferase( Fuc TIII)
Provide details,add link
130

Lymphocyte surface antigen CD38
S, 400 mg/L, PHO1
[240]

Lysosomal a-Mannosidase
S, 83 mg/L, native
[241]

Lysozyme
S, 300 mg/L, α-MF or native signal, active
405, 420, 562

Mast cell tryptase
S, 6.5 mg/L, α-MF
[242, 243]

Membrane Type 1 Matrix Metalloproteinase
S, 25mg/L, α-MF, active
423, 542

MHC class II heterodimers (soluble form/HLA-DR2)
S, 400 mg/L, α-MF
[244]

Midkine
S, α-MF, native, PHO1, 640 mg/L, active
544, 567, 568

Monoclonal single chain Fv
S, 50 mg/L, α-MF
[245]

Monocyte chemoattractant protein-1 (MCP-1)
S, 100 mg/L, native or α-MF
[246]

Monocyte chemotactic protein 3 (hMCP-3)
S, 1 mg/L, PHO1
[247]

Multidrug resistance protein 1
I, active
529, 570

Neural cell adhesion molecule (NCAM)
S, 50 mg/L, PHO1
[248]

Neutral endopeptidase(neprilysin)
S, 10 mg/L, α-MF
402

NonO nucleic acid binding protein
I (endoplasmic reticulum)
[249]

Oncostatin M(OSM)
S, 50 mg/L, α-MF, active
416

mu-Opioid receptor
undefined, α-MF
[199]

Pancreatic a-amylase
S, 20 mg/L, α-MF
[250]

Pancreatic triglyceride lipase
S, 75 ml/L, PHO1
[251]

Papain nitrile hydratase
S, 5 mg/L, α-MF
[252]

Group-V Phospholipase-A2
S, α-MF, active
450

Placental alkaline phosphatase (PLAP)
S, 2 mg/L, PHO1
[253]

Placental protein-14 (PP-14)
S, α-MF
[254]

Single-chain urokinase-type plasminogen activator
S, 5 mg/L, pre Mucor pusillus
rennin (MPR) signal
[273]

Sonic hedgehog-Fc fusion protein
S, α-MF, active
508

P-glycoprotein mdr1
I, active
570

Plasminogen kringle 1-4
S, 17 mg/L, PHO1
[255]

Plasminogen kringles 1-4, angiostatin protein
S, 10% total protein, PHO1
[256]

Plasminogen kringle 2
S, 100 mg/L, α-MF, functional
419

Presenilin I
I, α-MF, active
368

Procarboxypeptidase A2
S, 180 mg/L, α-MF
[257]

Procathepsin B
S, 20 mg/L, a-MF
[258]

Procolipase
S, 30 mg/L, native
[259]

Prolyl 4-hydroxylase
I, α-MF, active
437

Prostate-specific antigen
S, 1.5 mg/L, α-MF, active
534, 566

Protein kinase C interacting protein 1 (PKCI-1)
I, 0.25 mg/L
[260]

Type 2A Protein Phosphatase, subunit PR65/A

Subunit PP2Ac
I, 5 mg/l

I, 100 ug/l(toxic?)

Active complex
433

Proteinase 3, Wegener's antigen
S, 670 mg/L, α-MF
[261]

Proteinase inhibitor 8
I, 15% total protein
[262]

Serum albumin(wild type and disease variants)
S, 3 g/L, native, in Phase III trials
[265,266,
267, 268, 404, 408, 426, 571

Serum transferrin N-Lobe
S, 240 mg/L, α-MF
[269,270,271]

Sex steroid-binding protein
S, 4 mg/L, α-MF
[272]

Galβ1,3GalNAc α2,3-sialyltransferase
S, PHO1, inactive
429

α-2,6-sialyltransferase
S, α-MF, active
415

Sonic hedgehog variants
S, α-MF, much is trauncated, inactive
438

Sonic hedgehog fused to murine and rat IgG1 hinge and Fc
S, α-MF, active
508

Thrombomodulin
S
[274]

Tissue factor extracellular domain
S, 10 mg/L, PHO1
[275]

Tissue kallikrein
S, 30 mg/L, α-MF
[276, 277]

Tissue-type plasminogen activator kringle 2 domain
S, 170 mg/L, α-MF
[278,279,280, 281,282,283]

TRAIL tumor necrosis factor
S, 25 mg/L, α-MF, functional
436

Transforming growth factor β receptor extracellular domain
S, 10 mg/L, α-MF
[284]

Trypsinogen
S, 500 mg/L, α-MF,
547

Tumor necrosis factor αTNF
I, 10 g/L
[285, 286]

Type 1 plasminogen activator inhibitor (PAI-1)
S, 3 mg/L, α-MF
[287]

UDP-galactose-4-epimerase
I, 800 mg/L, α-MF, functional
435

UDP-glucoronosyltransferase (UDP-1A6)
I, active
422

Urokinase-type plasminogen activator-annexin V chimeras
S, 600 IU/ml, pre Mucor
pussilus rennin (MPR) signal
[289]

Vascular endothelial growth factor (VEGF165)
S, 40 mg/L , PHO1 or α-MF, active
[290], 565

Viruses

A/VICTORIA/3/75 Influenza virus neuraminidase head domain
S, 3 mg/ml, α-MF
[291, 292]

Bovine Herpes virus-1 glycoprotein D
S, 20 mg/L, α-MF
[293, 294]

Dengue virus type 1 structural gene recombinant E protein
S, PHO1, prM virus signal sequence
[295]

Dengue virus type 2 envelope protein fusion
I, 1 mg/50g cells; S, 100 mg/L, α-MF, functional
553, 557

Equine herpesvirus1 glycoprotein D
S, 100 mg/L, α-MF, active
555

Hepatitis B virus surface antigen
I, 400 mg/L; S 6ug/100 OD
[296, 297], 551

Hepatitis B virus surface antigen-HIV gp41 epitope chimera
I
[298]

Hepatitis B X protein
I, RSV-CAT reporter
444

Hepatitis C E.1.339 polypeptide
I, functional
440

Hepatitis C virus core protein
I, functional
554

Hepatitis E virus ORF3
I
[299]

HSV-1 and –2 glycoproteins D
S, 300 mg/L, α-MF, functional
552

Human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein, gp120 (ENV)
S, 20 mg/L, α-MF
[300]

HIV-1 gp41 ectodomain
S, α-MF, active
446

HIV-2 external glycoprotein mutant
S, PHO1, intact
659

Influenza virus RNA Polymerase
I, 2mg/L, α-MF, active
442

Mumps virus nucleocapsid protein
I, 2.1 mg/g biomass, active
556

Newcastle disease virus head protein
S, α-MF
445

Polyomavirus Large T antigen
I, 0.5 mg/L
[301]

Reovirus Lambda 1 core protein
I, 0.8 mg/L
[302]

Reovirus sigma 1 protein
I
[303]

Vaccinia virus complement control protein
S, 3 mg/L, α-MF
[304, 443]

I = Intracellular (with subcellular location),
S = Secreted,
undefined = Secreted to plasma membrane.
Amounts are highest reported for particular protein.
Signal sequences: a-MF (S. cerevisiae a-mating factor);
PHO1 (P. pastoris acid phosphatase);
SUC2 (S. cerevisiae invertase).