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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD16a / FCGR3A Protein (ECD, 176 Phe) (His & AVI Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25190.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:Human CD16a / FCGR3A Protein (ECD, 176 Phe) (His & AVI Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human CD16a His & AVI (Cat:10389-H27H) at 2 μg/ml (100 μl/well) can bind Human IgG1 (Cat:10702-HNAC), the EC50 of Human IgG1 is 0.4-1.2 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gly 17
蛋白分子量:The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 46 kDa due to glycosylation.
蛋白NP号:P08637-1
蛋白氨基酸序列:Met1-Gln208,176F
蛋白标签:C-AVI & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Cell Surface Immunofluorescence Staining Protocol
Staining Buffer at 5-10 x 10 cells/ml and distribute 100 μl/tube cells/tube) into 12 X 75 mm plastic tubes. 3. Block Fc-Receptors: Reagents that block Fc receptors may be useful for reducing nonspecific immunofluorescent staining
,亲合力增高Kd为10-8M。IgG与Clq结合点位于CH2功能区中最后一个β折叠股318~322位氨基酸残基(Glu-x-Lys-x-Lys)。IgM倍以上。人类天然的抗A和抗B血型抗体为IgM,血型不符合引韦的输血反应发生快而且严重。 2.凝聚的IgA、IgG4和IgE等可通过替代途径活化补体。 (三)结合Fc受体 不同细胞表面具有不同Ig的Fc受体,分别用FcγR、FcεR、FcαR等来表示。当Ig与相应抗原
,亲合力增高Kd为10-8M。IgG与Clq结合点位于CH2功能区中最后一个β折叠股318~322位氨基酸残基(Glu-x-Lys-x-Lys)。IgM倍以上。人类天然的抗A和抗B血型抗体为IgM,血型不符合引韦的输血反应发生快而且严重。 2.凝聚的IgA、IgG4和IgE等可通过替代途径活化补体。 (三)结合Fc受体 不同细胞表面具有不同Ig的Fc受体,分别用FcγR、FcεR、FcαR等来表示。当Ig与相应抗原
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