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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Trypsin-3 / PRSS3 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:Human Trypsin-3 / PRSS3 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human PRSS3 isoform c (P35030-3) (Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (AnaSpec, Catalog#27114) . The specific activity is >4,000 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 16
蛋白分子量:The secreted recombinant pro form of the human PRSS3 consists of 243 amino acids and predictes a molecular mass of 26.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRSS3 is approximately 33 kDa due to glycosylation.
蛋白NP号:P35030-3
蛋白氨基酸序列:Met1-Ser247
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Lei, Z; et al. Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases Activities. Anal. Chem., Pubmed: 28516765
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