Coronavirus spike重组蛋白|Recombinant HKU1(isolate N1)Spike/S1 Protein(S1 Subunit,His Tag)
- ￥1010 - 5820
- Sino Biological
- 北京
- 40021-V08H
- 2025年09月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant HKU1(isolate N1)Spike/S1 Protein(S1 Subunit,His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格： | 5.00 µg | 产品价格： | ￥1010.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 50.00 µg | 产品价格： | ￥4130.0 |
| 规格： | 100.00 µg | 产品价格： | ￥5820.0 |
冠状病毒HCoV-HKU1 Coronavirus spike蛋白|Coronavirus spike protein|Coronavirus spike(HCoV-HKU1, C-His)（产品说明）
蛋白名称：Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建：A DNA sequence encoding the human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit) (YP_173238.1) (Met 1-Arg 760) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 16
蛋白分子量：The recombinant human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit, His Tag) consists of 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：YP_173238.1
蛋白氨基酸序列：Met1-Arg760
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验1, Pierce CA, et al. Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients.Science translational medicine, PubMed ID: 32958614
2, Bertoglio F, et al. SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.Nature communications, PubMed ID: 33707427
3, Aydillo T, et al. Immunological imprinting of the antibody response in COVID-19 patients.Nature communications, PubMed ID: 34145263
4, Ravindran R, et al. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses.PloS one, PubMed ID: 34242356
5, Longworth J, et al. An antigen microarray protocol for COVID-19 serological analysis.STAR protocols, PubMed ID: 34462737
6, Denninger V, et al. Microfluidic Antibody Affinity Profiling Reveals the Role of Memory Reactivation and Cross-Reactivity in the Defense Against SARS-CoV-2.ACS infectious diseases, PubMed ID: 35352558
7, Anderson EM, et al. SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations.Cell reports, PubMed ID: 36261003
coronavirus, porcine hemagglutinating encephalomyelitis virus, equine coronavirus, bovine coronavirus (BCoV), and wild-ruminant coronaviruses. The presence of a hemagglutinin-esterase (HE) surface glycoprotein in addition to the viral spike protein
【分享】两本糖生物学方面的 HUMANA Protocols
between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and the use of two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity
5位院士为代表，超12篇CNS大作，5月新冠研究汇总——全方位围攻新冠病毒，值得收藏
病毒所石正丽团队与福建师范大学欧阳松应教授在 bioRxiv 上发表题为Evolutionary arms race between virus and host drives genetic diversity in bat SARS related coronavirus spike genes 的研究。结果表明，SARSr-CoV Spike蛋白和中华菊头蝠 ACE2 可能是随着时间的推移共同进化的，并经历来自彼此的选择压力，引发军备竞赛（Arms race）的协同进化关系，进一步证明中华菊
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