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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-15383R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-CD39/FITC
- 抗体名:
Anti-CD39/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat, Pig, Cow, Sheep,
- 适应物种:
Human, Mouse, Rat, Pig, Cow, Sheep,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-CD39/FITC |
| 中文名称 | FITC标记的CD39/ENTPD3抗体 |
| 别 名 | entpd1; ATPDase; CD 39; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto apyrase; Ecto ATP diphosphohydrolase; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTP1_HUMAN; ENTPD 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Sheep, |
| 产品应用 | Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD39/entpd1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Members of the ecto-nucleoside triphosphate diphosphohydrolase (eNTPDase) protein family are glycosylated enzymes that hydrolyze nucleoside 5’-triphosphates and 5’-diphosphates in extracellcular space. The enzymatic activities of eNTPDase are dependent on cations such as magnesium and calcium. Members of this protein family differ in their affinities for triphosphates versus diphosphates as substrate material. CD39L3, also known as Ectonucleoside triphosphate diphosphohydrolase 3 or NTPDase 3, is a 529 amino acid multi-pass membrane protein that has a three-fold preference for hydrolysis of ATP over ADP. Both calcium and magnesium serve as cofactors for CD39L3’s extremely active extracellular nucleotide hydrolysis site. CD39L3 is expressed in spleen, prostate, brain and pancreas, though moderate to low expression is observed in most tissues, with the exception of liver and peripheral blood leukocytes. Function: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Subunit: Homodimer; disulfide-linked (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein (Potential). Tissue Specificity: Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only. Post-translational modifications: The N-terminus is blocked. Palmitoylated in the N-terminal part. Similarity: Belongs to the GDA1/CD39 NTPase family. Database links: Entrez Gene: 953 Human Entrez Gene: 12495 Mouse Entrez Gene: 64519 Rat Omim: 601752 Human SwissProt: P49961 Human SwissProt: P55772 Mouse SwissProt: P97687 Rat Unigene: 576612 Human Unigene: 2824 Mouse Unigene: 17491 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验抗大鼠波形蛋白单克隆抗体,l:200稀释度),4℃过夜或37℃孵育30分钟,BS漂洗3次,每次5分钟; (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1:100),37℃避光孵育30分钟,PBS漂洗3次,每次5分钟; (8)Hoechst 333442复染细胞核15~20分钟(不是必需步骤); (9)甘油磷酸缓冲液封片,荧光显微镜下镜观察; (10)结果分析:波形蛋白免疫反应阳性细胞胞质呈现绿色荧光,如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
中。5. 用无菌 PBS 将上述锥形试管填满(至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下,以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞(BD 产品目录号:555899,10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。)8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中(PBS 含有 5% 正常人血清)。9. 加入抗体:CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
蛋白TFAR19蛋白的表达和细胞定位分析 TFAR19(PDCD5)是由本研究室在国际上首先报导的一个拥有自己知识产权的人类新基因,前期的功能研究表明,它是促进细胞凋亡的增强剂。利用荧光素(FITC)标记的TFAR19单克隆抗体为探针,对细胞凋亡过程中TFAR19蛋白的表达水平及定位研究发现,凋亡早期TFAR19表达水平增高并出现快速核转位现象,伴随着细胞核形态学的变化,持续较长时间,在凋亡小体中仍然可见。同时我们发现,凋亡早期TFAR19蛋白的核转位早于磷脂酰丝氨酸(PS)外翻和细胞核DNA的片段
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