- ¥780 - 2200
- LMAI Bio
- LM-7686R
- 进口/国产
- 2025年12月18日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-7686R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
GPR55
- 抗体名：
蛋白偶联受体55抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from human GPR55:141-240/319 <Extracellular>
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | GPR55 |
| 中文名称 | 蛋白偶联受体55抗体 |
| 别 名 | GPCR GPR55; G protein coupled receptor 55; G protein-coupled receptor 55; G protein-coupled receptor 55; GPCR GPR55; GPR 55; GPR55; Probable G protein coupled receptor 55; GPR55_HUMAN. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 心血管 细胞生物 信号转导 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR55:141-240/319 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries. Function: May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9Y2T6 Gene ID: 9290 Database links: Entrez Gene: 9290 Human Omim: 604107 Human SwissProt: Q9Y2T6 Human Unigene: 114545 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-GPR55 (bs-7686R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD  Sample: K562 Cell Lysate at 40 ug Primary: Anti- GPR55 (bs-7686R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD  Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (bs-7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.  Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (bs-7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. |
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文献和实验和抗体配对最早于20世纪80年代被发现，当时科学家将Rho1D4抗体交联包被在琼脂糖凝胶上，用来纯化通过猴肾细胞表达的牛视紫红质。1,2从那时起，Rho1D4系统开始被广泛的用于小量膜蛋白提取的研究中，包括GPCR（G蛋白偶联受体），ATP结合盒转运蛋白（ATP-binding cassette transporter），溶质反向转运体和以及一种含四个跨膜域的膜蛋白。3） Rho1D4系统的优势和用途高纯度Rho1D4系统包含：标签、抗体偶联亲和基质（琼脂糖或磁珠等）以及洗脱多肽。Rho
rebeccagold 大家好。 最近在做建一个高表达某G蛋白偶联受体的CHO细胞株，载体是pEGFP-C1。 稳定筛选后，细胞的荧光表达很好。 但奇怪的问题是，该受体定位出现了问题。原本该定位在膜的G蛋白偶联受体，现在基本上呈全细胞分布，而且核尤其亮。百思不得其解。 请各位战友指点。 附图为该细胞的Confocal图，没有染核。 多谢大家啦！ kern6549 会不会是该受体
能减少失败的概率，所以同学们做实验时也不要过于有心里负担，怕这怕那的，要保持一个自然的心态，否则容易抑郁。今天我们就来看一看生物信息学的各种神器是如何来帮助我们的 Western blot 实验。大家在查找抗体的时候有没有看见免疫原的序列和这两个图标呢？这个意思就是说厂家在生产这个抗体的时候，先是合成了一段肽链，这个肽链的氨基酸序列是从蛋白质序列里面摘录的，下面 blast with 意思就是分别和 NCBI 的数据库或 Expasy 的蛋白质数据库里的蛋白质氨基酸序列进行比对。这个 blast
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