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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-15383R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
CD39
- 抗体名:
CD39/ENTPD3抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Pig, Cow, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human CD39/entpd1 :101-200/510 <Extracellular>
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | CD39 |
| 中文名称 | CD39/ENTPD3抗体 |
| 别 名 | entpd1; ATPDase; CD 39; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto apyrase; Ecto ATP diphosphohydrolase; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTP1_HUMAN; ENTPD 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD39/entpd1 :101-200/510 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Members of the ecto-nucleoside triphosphate diphosphohydrolase (eNTPDase) protein family are glycosylated enzymes that hydrolyze nucleoside 5’-triphosphates and 5’-diphosphates in extracellcular space. The enzymatic activities of eNTPDase are dependent on cations such as magnesium and calcium. Members of this protein family differ in their affinities for triphosphates versus diphosphates as substrate material. CD39L3, also known as Ectonucleoside triphosphate diphosphohydrolase 3 or NTPDase 3, is a 529 amino acid multi-pass membrane protein that has a three-fold preference for hydrolysis of ATP over ADP. Both calcium and magnesium serve as cofactors for CD39L3’s extremely active extracellular nucleotide hydrolysis site. CD39L3 is expressed in spleen, prostate, brain and pancreas, though moderate to low expression is observed in most tissues, with the exception of liver and peripheral blood leukocytes. Function: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Subunit: Homodimer; disulfide-linked (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein (Potential). Tissue Specificity: Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only. Post-translational modifications: The N-terminus is blocked. Palmitoylated in the N-terminal part. Similarity: Belongs to the GDA1/CD39 NTPase family. SWISS: P49961 Gene ID: 953 Database links: Entrez Gene: 953 Human Entrez Gene: 12495 Mouse Entrez Gene: 64519 Rat Omim: 601752 Human SwissProt: P49961 Human SwissProt: P55772 Mouse SwissProt: P97687 Rat Unigene: 576612 Human Unigene: 2824 Mouse Unigene: 17491 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Blank control: Raji cells (blue). Primary Antibody:Rabbit Anti- CD39 antibody(bs-15383R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-15383R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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