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- 雅酶
- PS103S
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 保存条件：
常温
- 规格：
500mL
TBS/Tween Buffer (10×)
本产品常温运输；保存于室温，保质期1年。
货号规格
goodPS103S 500mL
产品特点
g
d即用型试剂，大大提高实验效率；
g
d可能是市场上性价比最高的常用缓冲液。相关论文
good1. Yuliantie, E., Dai, X., Yang, D., Crack, P. J., & Wang, M. W. (2018). High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway. Acta pharmaceutica sinica B, 8(6), 889-899. （IF 11.413）
good2. Zhou, F. L., Li, S. C., Zhu, Y., Guo, W. J., Shao, L. J., Nelson, J., ... & Xu, J. B. (2019). Integrating yeast chemical genomics and mammalian cell pathway analysis. Acta Pharmacologica Sinica, 40(9), 1245-1255. （IF 6.150）
good3. Wang, Y. Z., Liu, X., Way, G., Madarha, V., Zhou, Q. T., Yang, D. H., ... & Wang, M. W. (2020). An in vitro Förster resonance energy transfer-based high-throughput screening assay identifies inhibitors of SUMOylation E2 Ubc9. Acta Pharmacologica Sinica, 1-10. （IF 6.150）
good4. Wang S, Ma X, Guo J, et al. DDIT3 antagonizes innate immune response to promote bovine alphaherpesvirus 1 replication via the DDIT3-SQSTM1-STING pathway[J]. Virulence, 2022, 13(1): 514-529. （IF 5.882）
good5. Lin, G., Liu, Q., Dai, A., Cai, X., Zhou, Q., Wang, X., ... & Wang, M. W. (2020). Characterization of a naturally occurring mutation V368M in the human glucagon receptor and its association with metabolic disorders. Biochemical Journal, 477(13), 2581-2594. （IF 3.857）
good6. Wu, J., Zhao, F. T., Fan, K. J., Zhang, J., Xu, B. X., Wang, Q. S., ... & Wang, T. Y. (2019). Dihydromyricetin Inhibits Inflammation of Fibroblast-Like Synoviocytes through Regulation of Nuclear Factor-κB Signaling in Rats with Collagen-Induced Arthritis. Journal of Pharmacology and Experimental Therapeutics, 368(2), 218-228. （IF 4.030）
good7. ZHONG, Li, et al. W2476 represses TXNIP transcription via dephosphorylation of FOXO1 at Ser319. Chemical Biology & Drug Design, 2021, 97.5: 1089-1099.（IF 2.817）
good8. You K, Liu Y, Chen L, et al. Radix ranunculus temate saponins sensitizes ovarian cancer to Taxol via upregulation of miR‑let‑7b[J]. Experimental and Therapeutic Medicine, 2022, 23(5): 1-9.（IF 2.447）
good9. Li, X., Zhang, W., Liang, L., Duan, X., Deng, J., & Zhou, Y. (2020). Natural product‑derived icaritin exerts anti‑glioblastoma effects by positively modulating estrogen receptor β. Experimental and Therapeutic Medicine, 19(4), 2841-2850. （IF 2.447）
good10. HAN, Shuguang, et al. Maternal obesity accelerated nonalcoholic fatty liver disease in offspring mice by reducing autophagy. Experimental and Therapeutic Medicine, 2021, 22.1: 1-10. （IF 2.447）
good11. ZHENG, Yuming, et al. Genetic variations in DNA repair gene NEIL1 associated with radiation pneumonitis risk in lung cancer patients. Molecular Genetics & Genomic Medicine, 2021, e1698.（IF 2.183）
good12. HUANG, Yang, et al. Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension. Journal of International Medical Research, 2021, 49.3: 03000605211002379.（IF 1.671）
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文献和实验；Aprotinin,leupeptin,pepstatin:1 microgram/ml each；Na3VO4: 1 mM；NaF: 1 mM 3. 1X SDS 样品缓冲液：62.5 mMTris-HCl(pH 6.8 于 25° C), 2% w/v SDS, 10%甘油,50 mMDTT, 0.01% w/v溴酚蓝 4. 转移缓冲液：25 mM Trisbase, 0.2 M 甘氨酸, 20%甲醇 (pH 8.3) 5. 10X Tris缓冲盐 (TBS)：准备1L
1、试剂耗材的准备：（1）转膜缓冲液（48 mM Tris-HCl，39 mM 甘氨酸，0.037% SDS，20%甲醇）：（2）10 × 丽春红染液：（3）TBS 缓冲液：（4）TBST 缓冲液（含20%Tween20 的TBS 缓冲液）：（5）封闭液（含5%脱脂奶粉的TBST 缓冲液）：（6）一抗、二抗及抗体稀释液：参照说明书，以封闭液或TBST 进行稀释。（7）底物显色液ECL，4℃ 避光保存，现用现配。（8）显影液和定影液用水稀释10 - 20 倍于铝盒中，可多次使用。室温避光存放
SDS-PAGE，蛋白转印，western Blot试剂配制
Technology Protoco） Wash Buffer TBS/T:1X TBS, 0.1% Tween-20[自配] Wash Buffer TBS/T:1000ml1×TBS,0.1%Tween-20,100ml 10×TBS+ 1ml Tween-20 + 900ml ddH2O（参照Cell signal Technology Protoco） Blocking Buffer:1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk。[自配
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