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SK-N-DZ 人成神经瘤细胞

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  • 2025年12月18日
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    上海复祥生物科技有限公司
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    • 供应商：

      复祥生物

    • 品系：

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    • 组织来源：

      人

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    • 物种来源：

      人

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    • 是否是肿瘤细胞：

      是

    • 器官来源：

      人

    • 运输方式：

      常温

    • 生长状态：

      正常

    • 库存：

      大量

    • 规格：

      株

    CRL-2149 SK-N-DZ 人成神经瘤细胞-骨髓，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！

    CRL-2149 SK-N-DZ 人成神经瘤细胞-骨髓 的详细介绍
    CRL-2149 SK-N-DZ 人成神经瘤细胞-骨髓
    ATCC® Number: CRL-2149™ Price: $338.00
    Designations: SK-N-DZ
    Depositors: C Helson
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: brain
    Disease: neuroblastoma
    Derived from metastatic site: bone marrow
    Cell Type: neuroblast;
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Tumorigenic: Yes
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 12
    D13S317: 8,11
    D16S539: 9,11
    D5S818: 12
    D7S820: 12,13
    THO1: 6,9
    TPOX: 8
    vWA: 16,18
    Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen
    Age: 2 years
    Gender: female
    Ethnicity: Caucasian
    Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.
    Retinoic acid induces differentiation in this line.
    Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.
    Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.
    The cells exhibit moderate MDR1 expression.
    Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
      • Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
        • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            • Add appropriate aliquots of the cell suspension to new culture vessels.
              • Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020
    References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792
    22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824
    23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694

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    • 处理的瘤细胞作为肿瘤疫苗

      处理的瘤细胞作为肿瘤疫苗       肿瘤疫苗包括灭活的自体肿瘤细胞、提取的肿瘤抗原和人工合成的肿瘤抗原肽，通过给患者免疫接种，激发患者自身对肿瘤细胞的特异性免疫应答，清除肿瘤而不损伤周围正常细胞。肿瘤疫苗还可诱发免疫记忆细胞，产生长期的免疫效应，防止肿瘤的转移和复发。是一种理想的特异性主动免疫治疗手段。   肿瘤疫苗激活T细胞作用机制 肿瘤细胞表达的肿瘤抗原经APC加工处理，通过MHCI类和Ⅱ类分子提呈至细胞表面，分别激活CD

    • 肿瘤细胞因子基因疗法

      肿瘤细胞因子基因疗法       细胞因子基因疗法是将细胞因子或其受体基因通过一定技术方法导人体内，使其在体内持续表达并发挥治疗效应。目前已有多项细胞因子基因疗法试用于临床治疗恶性肿瘤、感染、自身免疫病等。   基因治疗 细胞因子基因疗法是将细胞因子或其受体基因通过一定技术方法导入体内，使其在体内持续表达并发挥治疗效应。  例如，利用凝血因子Ⅷ基因疗法治疗血友病，将荷载凝血因子Ⅷ基因的病毒载体注射入血友病人血液中，可持续表达凝血因子Ⅷ，以达到治疗血友病的效果。  

    • 【求助】PI3K在不同肿瘤细胞的区别

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