HS-746T 人胃癌细胞

询价

价格询价
品牌 ATCC
产地美国
货号 HS-746T
更新日期 2024年11月12日

上海复祥生物科技有限公司

12 年金牌会员

供应商：
ATCC
品系：
查询相关资料
组织来源：
人胃癌
相关疾病：
查询相关资料
物种来源：
人
免疫类型：
查询相关资料
是否是肿瘤细胞：
否
器官来源：
人
运输方式：
常温
年限：
2020
生长状态：
正常
库存：
大量

HTB-135 HS-746T 人胃癌细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！

HTB-135 HS-746T 人胃癌细胞系的详细介绍

HTB-135 HS-746T 人胃癌细胞系

ATCC^® Number:

HTB-135™

Price:

$323.00

Designations:	Hs 746T
Depositors:	RB Owens
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: stomach Disease: gastric carcinoma Derived from metastatic site: left leg
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	California, United States Isolation date: 1973
Tumorigenic:	Yes
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10 D16S539: 11 D5S818: 11,13 D7S820: 8 THO1: 9 TPOX: 8,10 vWA: 15,17
Cytogenetic Analysis:	This is a hypertriploid human cell line having the modal chromosome number of 77 occurring in 46% of cells. Cells with higher ploidies were found at 16.6%. The karyotype of this cell line is very complex. Between 45% and 55% of chromosomes in each cell had structural aberrations, including: der(4)t(1;4) (p13.1;q35); t(15q22q); del(2) (p13); der(7)t(7;?) (p22;?) [7p+]; t(7q22q?) and others. The t(15q22q), del(2), and t(7q22q?) (and several others) had two copies in most cells. There were two types of der(7): one was the 7p+ described above; the other, 7p++, was distinctly longer than N1. There were more than ten markers that were present in some cells only. One DM-like chromosome was found in most cells. Normal N7 and N21 were absent. One normal X and one or two normal Y chromosomes were detected.
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1-2 PGM3, 1
Age:	74 years adult
Gender:	male
Ethnicity:	Caucasian
Comments:	In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
References:	22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

文献和实验

相关实验

移动版：产品详情页

HS-746T 人胃癌细胞

HTB-135 HS-746T 人胃癌细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！

相关产品推荐更多 >

HSCT6 大鼠肝星状细胞

TIB-68 WEHI-3 小鼠血液细胞

VERO细胞非洲绿猴肾细胞CCL-81 ATCC原装进口

CRL-2755 EMT6 大鼠乳腺癌细胞

CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞

万千商家帮你免费找货

人在求购买到急需产品

询价发送成功

你可能感兴趣的产品

关于丁香通

公司信息

个人用户

企业机构

HS-746T 人胃癌细胞

HTB-135 HS-746T 人胃癌细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！

同类产品报价

你可能感兴趣的产品

相关产品推荐更多 >

HSCT6 大鼠肝星状细胞

TIB-68 WEHI-3 小鼠血液细胞

VERO细胞 非洲绿猴肾细胞CCL-81 ATCC原装进口

CRL-2755 EMT6 大鼠乳腺癌细胞

CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞

万千商家帮你免费找货

人在求购买到急需产品

询价发送成功

你可能感兴趣的产品

关于丁香通

公司信息

个人用户

企业机构

VERO细胞非洲绿猴肾细胞CCL-81 ATCC原装进口