SgeI (3 U/µL)

SgeI (3 U/µL)

描述

5'			Cm5	N	N	G	N9	↓		3'
3'			G	N	N	C	N13	↑		5'

SgeI cleaves DNA targets containing 5-methylcytosine on one or both DNA strands

Thermo Scientific SgeI restriction enzyme recognizes m5CNNG(9/13)^ sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

规格

Compatible Buffer:	Unique Buffer (10x Buffer SgeI)
Enzyme:	SgeI
Methylation Sensitivity:	Not CpG methylation-sensitive, Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Product Size:	250 units
Sensitive to Heat Inactivation:	Yes