- ￥2950
- lucigen
- CL9021K
- CL9021K
- 2025年10月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
CL9021K
- 英文名：
CircLigase™ II ssDNA Ligase
- 供应商：
上海研卉生物科技有限公司
- 保存条件：
1,000 U @ 100 U/uL
- 规格：
1,000 U @ 100 U/uL
CircLigase II ssDNA Ligase, 1000 U
- Intramolecular ligation of single-stranded DNA and RNA ends containing 5'-phosphates and 3'-hydroxyl groups
- ATP-independent, non-catalytic enzyme
- Circularises ssDNA 15 bases long
- Compatible with high temperature ligation reactions due to thermostability of CircLigase II enzyme
-
Product information
CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyses intramolecular ligation (i.e. circularisation) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. In contrast to T4 DNA Ligase and Ampligase™ DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA. Circular ssDNA molecules can be used as substrates for rolling-circle replication or rolling-circle transcription.
Applications
- Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments.
- Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays.
Linear ssDNA of >15 bases, including cDNA, is circularised by CircLigase II enzyme. Under standard reaction conditions, virtually no linear concatemers or circular concatemers are produced. In addition to its activity on ssDNA, CircLigase II enzyme also has activity in ligating a single-stranded nucleic acid having a 3´-hydroxyl ribonucleotide and a 5´-phosphorylated ribonucleotide or deoxyribonucleotide.
CircLigase II has greater activity and a new Reaction Buffer for improved ligation efficiency.
Figure 1. CircLigase II ssDNA Ligase converts linear ssDNA
Product Citations
Polidoros, A.N. et al. (2006) BioTechniques 41, 35. Shroff , H. et al. (2005) Nano Letters 5(7), 1509. Lin, C. et al. (2006) Angewandte Chemie 118, 7699. Korlach, J. et al. (2008) Proc. Natl. Acad. Sci. USA 105, 1176. McArthur, M. and Bibb, M.J. (2008) Proc. Natl. Acad. Sci. USA 105, 1020. Shroff , H. et al. (2008) Biophysical Journal 94, 2179. Kuhn, H. and Frank-Kamenetskii, M.D. (2008) Nucleic Acids Res. 36, e40. Murakami, T. et. al. (2008) Nucleic Acids Res. Doi:10.1093/nar/gkn1014
使用方法：Product specifications and usage
Unit Definition: One unit of CircLigase II enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase II Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton ® X-100.
10X Reaction Buffer: 0.33 M Tris-Acetate (pH 7.5), 0.66 M potassium acetate and 5 mm DTT.
Quality Control: CircLigase II ssDNA Ligase is free of detectable phosphatase, DNA exo- and endonuclease, and RNase activities.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Product Citations
- Polidoros, A.N. et al. (2006) BioTechniques 41, 35.
- Shroff , H. et al. (2005) Nano Letters 5(7), 1509.
- Lin, C. et al. (2006) Angewandte Chemie 118, 7699.
- Korlach, J. et al. (2008) Proc. Natl. Acad. Sci. USA 105, 1176.
- McArthur, M. and Bibb, M.J. (2008) Proc. Natl. Acad. Sci. USA 105, 1020.
- Shroff , H. et al. (2008) Biophysical Journal 94, 2179.
- Kuhn, H. and Frank-Kamenetskii, M.D. (2008) Nucleic Acids Res. 36, e40.
- Murakami, T. et. al. (2008) Nucleic Acids Res. Doi:10.1093/nar/gkn1014
on ligation of an adapter to the 3′ end of cDNA with bacteriophage TS2126 RNA ligase (CircLigase™). In the following PCR amplification, Illumina adapters and index sequences are introduced, thereby allowing amplicons to be pooled and sequenced on the standard
，当限量的那个引物耗光后，随后的 5-10 个循环就产生出 ssDNA，产生的 ds-DNA 与 ss-DNA 由于分子量不同，可以通过电泳分离。 ssDNA 产物量 一般对 100μl PCR 反应体系而言，当引物比率为 50pmo:0.5pmol，经过 30 个循环后，大约可产生 1-3pmol 的 ssDNA，ssDNA 的产量可用下列几种方法来估计： 1、在 ss-DNA 合成过程中，掺入 32P-dNTP。取 10% 的反应产物，经过琼脂糖电泳分离后，放射自显影。 2、取 5% 的反应物来走
A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template
two key enzymes, dUTPase (dut) and uracil N-glycosylase (ung) , will synthesize DNA with a small number of uracil bases substituted for thymine. Uracil containing single-stranded DNA (ssDNA) prepared from this dut − , ung − host strain can be used
技术资料暂无技术资料 索取技术资料
