- ￥1912
- LGC(Lucigen)
- RP8092H
- RP8092H
- 2025年10月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
CircLigase™ ssDNA Ligase
- 库存：
RP8092H
- 供应商：
上海研卉生物科技有限公司
- 规格：
400 U @ 20 U/uL
Dephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
- Dephosphorylates 5´-triphosphorylated RNA, such as primary RNA transcripts, but does not dephosphorylate 5´-capped mRNA
- Specific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA
- Heat Inactivated: Heat at 65 °C for 20 minutes to kill enzyme activity
- Flexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator 5´-Phosphate-Dependent Exonuclease
Unit Definition: One unit of RNA 5´ Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.
10X Reaction Buffer: 0.5 M HEPES-KOH (pH 7.5), 1 M NaCl, 10 mM EDTA, 1% β-mercaptoethanol, and 0.1% Triton® X-100.
Inactivation/Inhibitors: RNA 5´ Polyphosphatase is inhibited ~50% by 100 mM of inorganic phosphate (Pi) using ATP as a substrate.
Quality Control: RNA 5´ Polyphosphatase is free of detectable DNA exo- and endonuclease, and RNase activities.
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- 作者
- 内容
- 询问日期
文献和实验https://pubs.rsc.org/en/content/articlelanding/2023/RA/D3RA00026E
Saito, M., Xu, P., Faure, G. et al.
Nature 2023
Pregeljc, D., Skok, J., Vodopivec, T., Mencin, N., KruÅ¡iÄ, A., LiÄen, J., Nemec, K. Å ., Å trancar, A., & Sekirnik, R.
Biotechnology and Bioengineering 2022
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