- ￥1912
- LGC(Lucigen)
- RP8092H
- RP8092H
- 2025年10月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
CircLigase™ ssDNA Ligase
- 库存：
RP8092H
- 供应商：
上海研卉生物科技有限公司
- 规格：
400 U @ 20 U/uL
Dephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
- Dephosphorylates 5´-triphosphorylated RNA, such as primary RNA transcripts, but does not dephosphorylate 5´-capped mRNA
- Specific: Removes the γ and β phosphates from 5´-triphosphorylated and the β phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5´-capped RNA
- Heat Inactivated: Heat at 65 °C for 20 minutes to kill enzyme activity
- Flexible: Use for various applications such as 5´-RNA ligation-tagging using T4 RNA Ligase, analysis of 5´-end structure of RNA, and preparation of substrate RNAs for subsequent degradation with Terminator 5´-Phosphate-Dependent Exonuclease
Unit Definition: One unit of RNA 5´ Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.
10X Reaction Buffer: 0.5 M HEPES-KOH (pH 7.5), 1 M NaCl, 10 mM EDTA, 1% β-mercaptoethanol, and 0.1% Triton® X-100.
Inactivation/Inhibitors: RNA 5´ Polyphosphatase is inhibited ~50% by 100 mM of inorganic phosphate (Pi) using ATP as a substrate.
Quality Control: RNA 5´ Polyphosphatase is free of detectable DNA exo- and endonuclease, and RNase activities.
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- 作者
- 内容
- 询问日期
文献和实验https://pubs.rsc.org/en/content/articlelanding/2023/RA/D3RA00026E
Saito, M., Xu, P., Faure, G. et al.
Nature 2023
Pregeljc, D., Skok, J., Vodopivec, T., Mencin, N., KruÅ¡iÄ, A., LiÄen, J., Nemec, K. Å ., Å trancar, A., & Sekirnik, R.
Biotechnology and Bioengineering 2022
The term ribonuclease (RNase) is an imprecise term and is used to cover both enzymes that cause exonucleolytic cleavage and endonucleolytic cleavage of RNA. Exonucleases may cleave the RNA in 3′-5′ direction or vice versa
RNA Purification Protocol for 1-2 x 107 cultured cells
temperature. 8. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 3 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 9. Centrifuge at 3,000-5,000 x g for 10 minutes at room temperature
感染马铃薯、番茄等而抑制其生长病发育的毒。粒子是长515纳米，宽 13纳米的线状体，呈螺旋结构。核酸分子量为 2.1× 106 的单链 RNA，约占粒子重量的 6％。主要传染途径是接触和带毒马铃薯种薯流通引起的，也能由内生集壶菌（ Synchytriumendobit-icum）传播。寄主范围比较狭窄，仅限于茄科植物。
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