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- E3101K
- 2025年08月29日
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- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
2
- 规格：
1,000 Units
Plasmid-Safe™ ATP-Dependent DNase
Remove contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC preps
- Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)
- Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations
- Utilize whenever protecting circular dsDNA is important
Includes Plasmid-Safe™ 10X Reaction Buffer and 25-mM ATP Solution.
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文献和实验Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
MutSDNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance
Elucidation of the mechanisms of recognition of mispaired DNA by MutS and subsequent repair by the multicomponent mismatch repair machinery presents a fascinating challenge (1 ). ATP binding or hydrolysis are implicated in several steps
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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