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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Bsm DNA Polymerase, large fragment (8 U/µL)
- 规格：
1,600 units
描述
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is an equivalent to Bst DNA polymerase, which catalyzes 5'→3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii that has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°C. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.Highlights
• Thermophilic DNA polymerase with strong strand displacement activity
Applications
• Isothermal DNA amplification by the method of:
--Loop-mediated isothermal amplification (LAMP)
--Whole genome amplification (WGA)
--Ramification amplification (RAM)
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA
Note: Not suitable for use in PCR.
规格
| Polymerase: | Bsm DNA Polymerase |
|---|---|
| Product Size: | 1,600 units |
内容及储存
• 1.25 mL 10X Bsm Buffer
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文献和实验), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity
Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(1 . This enzyme was derived from E. coli . DNA Pol I which, in addition to polymerase activity, has both 3′–
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