EcoRV-HF RE-Mix is a ready to use 10X master mix combining the restriction enzyme, EcoRV-HF (NEB #R3195), NEBuffer, BSA and loading dye in a single tube. Simply add DNA and water and incubate for 15 minutes to perform the restriction digest.
Source: An E. coli strain that carries the cloned and modified (D19A, E27A) EcoRV gene from the plasmid J62 pLG74 (L.I. Glatman)
Enzyme Properties
Activity in NEBuffers:
NEBuffer 1:
Not Recommended
NEBuffer 2:
Not Recommended
NEBuffer 3:
Not Recommended
NEBuffer 4:
Not Recommended
Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Impaired by some combinations of overlapping
Survival in a Reaction: (+) Intermediate activity. Suitable for extended digestion, but < 8 hours. More information about: Extended Digests with Restriction Enzymes
Reaction & Storage Conditions
Incubate at 37°C.
Unit Definition: One reaction is defined as the amount of RE-Mix that will digest 1 μg of DNA in 15 minutes at 37°C in a total reaction volume of 20 μl.
Reaction Conditions: 1X EcoRV-HF RE-Mix and DNA in a total reaction volume of 20 μl. Incubate at 37°C for 15 minutes.