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- 文献和实验
- 技术资料
- 英文名:
MagAttract PowerWater DNA/RNA Kit (384)
- 规格:
盒
Qiagen 27800-4-EP MagAttract PowerWater DNA/RNA Kit (384)
The MagAttract PowerWater DNA/RNA Kit is a magnetic bead-based nucleic acid isolation kit optimized for use with the Eppendorf® epMotion® 5075 TMX platform. It is also compatible with other automated platforms with similar features.
The MagAttract PowerWater DNA/RNA Kit can be used for automated isolation of microbial DNA and RNA from any liquid or air sample that is concentrated on a filter membrane. Both 25 mm and 47 mm filter membranes made of a variety of materials can be processed with this kit, which employs Inhibitor Removal Technology® (IRT) to remove PCR inhibitors released during the extraction process. Additionally, a novel, proprietary magnetic bead system is used to isolate nucleic acids without binding residual contaminants. The result is inhibitor-free DNA and RNA that is ready to use in demanding downstream applications, including PCR, qPCR, RT-qPCR and next-generation sequencing (NGS).
The MagAttract PowerWater DNA/RNA Kit has been used to isolate DNA and RNA from a variety of different membrane types used for air and water sampling
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文献和实验DNA纯度曲线和凝胶电泳结果。图3B展示最终DNA的吸光值。值得注意的是,竞争对手试剂盒提取的DNA A260读数较高,表面上看起来得率是PowerSoil® Kit(P)的两倍,然而进一步的凝胶电泳能看到多出部分的DNA源于大量腐植酸污染(低230/260比值加上高340读数,图3B),以及回收的大量降解RNA(图3A)。后面的PCR扩增再次确认存在抑制因子。 再来一个实验对比我们的两款试剂盒效果;一款含IRT技术(PowerWater®),另一款不含IRT技术
仪以及进行了关于设计和操作的讨论。自动化装置性质AECOM点样仪,Albert,产生高密度的分隔的矩阵,矩阵包括cDNA、基因组DNA或其他类似的生物物质。机械的基本组成有计算机控制的三轴向的机械手和独特笔尖装置。设计特点机械手被设计成能自动从96或384孔的微量滴定板中选取样本,12支点样笔同时升起,每个点样笔收集了250~500nl溶液,在每块玻片上放置0.25-1nl,产生的点的大小范围直径为100~150μm。机械手是由设置好的程序控制的,能进行连续的点样,每一点避免与相邻的点接触,每点的中心
RT2 Profiler PCR Arrays新手小技巧及注意事项
给QIAGEN技术人员,我们会帮您分析您的RNA样品是否适合进行PCR array。cDNA合成:我推荐以1 ug的总RNA为模板,用RT2 First Strand Kit (Cat # 330401)试剂盒进行cDNA合成。根据我们的经验这是最佳的起始反应用量。假如RNA量不够,起始总RNA样品量最少可以减至400 ng,不过必须保证RNA的质量足够好;假如连400 ng的RNA样品都无法保证,请联系QIAGEN技术人员,您可能需要进行预扩增。请使用相同起始量的RNA进行cDNA合成及后继实验,以保证
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