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Intended Use
The Aquadien DNA Extraction and Purification Kit is used for reliable high-yield bacterial DNA extraction and purification from water samples to enhance sensivity and reproducibility of real-time PCR analysis. The kit must be used prior to real-time PCR assays for optimal results.
Key Benefits
- Developed for efficient bacterial DNA extraction from all water samples, aerosols, and biofilms
- Allows enhanced detection of bacteria in water samples, including Pseudomonas aeruginosa, Salmonellaspp., and E. coli
- Designed to work with iQ-Check® Legionella Real-Time PCR Kits or other real-time PCR analysis
- Optimized extraction from 1 L of water per sample
- Rapid detection and quantitation of pathogenic bacteria (24 hr)
- Standardized protocol for routine monitoring of drinking water, swimming pools, whirlpools, industrial water distribution systems, and other water sources
- AFNOR/ISO validated by AFNOR Certification according to the NF T90-471 and ISO TS 12869 standards
Principle
The protocol uses alkaline thermal lysis after tangential microfiltration (cross flow filtration), which reduces clogging of membrane pores during sample concentration.
Additional Information
Use the Aquadien W2 Wash Solution (3578119) for samples that clog the membrane and for samples containing PCR inhibitors.
Ordering Information
The Aquadien DNA Extraction and Purification Kit (3578121) contains the components for processing 96 samples.
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文献和实验糖凝胶上检测分析。如图一所示 图一:2.2 超声破碎后,8000g,30秒,4°C,离心。将上清移入新的管子中。开始准备进行染色质免疫共沉淀(IP)。2.3 每份超声样品取50ul,这时的样品标记为INPUT,用于定量DNA浓度和作为PCR的空白对照。超声后的染色质应立刻冻入液氮中或者储存于-70°C可储存2个月。避免多次反复冻融。3检测DNA浓度1INPUT样品用于为后来的IP样品计算DNA浓度。DNA可采用PCR纯化试剂盒(加入70ul洗脱液,接着进入3.2a)或者采用酚/氯仿抽提(加入350ul
1、外泌体提取纯化试剂盒如何保存? 室温保存(10℃~30℃),无需低温保存。温度过低时 ECS 试剂会出现冻结状态,此时用 65℃ 水浴 1 h 进行解冻即可。 2、外泌体提取纯化试剂盒能否分离纯化 200-1000 nm 直径的囊泡? 不能,本试剂盒不适用于直径大于 200 nm 囊泡的分离。粒径在 200 nm 以上的属于大囊泡,而非外泌体。 3、样品在提取外泌体之前是否可以低温保存? 可以。长期保存请放置于 -80℃,无需加冻存液;短期保存(1-2 天内)则放置于 4℃ 即可
专用间充质干细胞培养基,细胞培养24小时后,再替换为完全的外排体专用间充质干细胞的进行。 20、关于外泌体红色荧光标记染料(PKH26 / PKH67 )相关实验事项注意事项 a、建议用于染料标记的外泌体原始浓度达到0.5~1μg/μL。外泌体浓度过低实验失败风险较高。 b、外泌体染色步骤中去除游离染料的第4、5步必备,以色列游离染料对更新实验的干扰。这一步即将重新去除外泌体,所有外泌体去除封闭方式均适用。 c、去除游离染料的方法是按照外泌体提取方法再次提取外泌体,推荐umibio外泌体提取纯化试剂盒
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