Flow cytometric analysis of Human Th1/Th2/Th17 Staining Kit. The staining pattern of IFN-g , IL-4 and IL-17A on resting heparin anticoagulated blood (A0, B0 and C0). PMA/Ionomycin stimulated heparin anticoagulated blood (A1, B1 and C1). Dot plot analysis are derived from gated CD3+/CD8- cells population. Flow cytometry was performed on a Thermo Fisher Attune NxT. The health status of the volunteers is unclear.