Flow cytometric analysis of Human Th17 Staining Kit. The staining pattern of IL-17A on resting heparin anticoagulated blood (A0) and PBMCs from EDTA anticoagulated blood (B0). PMA/Ionomycin stimulated heparin anticoagulated blood (A1) and PBMCs from EDTA anticoagulated blood (B1). Dot plot analysis are derived from gated CD3+/CD8- cells population. Flow cytometry was performed on a Thermo Fisher Attune NxT. The health status of the volunteers is unclear.