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Lonza细胞核转染试剂盒，SF Cell Line 4D-

NucleofectorTM X Kit L
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  • ¥6315
  • LONZA
  • 美国
  • V4XC-2024
  • 2025年12月16日
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    Lonza细胞核转染试剂盒，SF Cell Line 4D-NucleofectorTM X Kit L
    Catalog #: V4XC-2024
    For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (100 µL format).

    Product Overview

    The SF Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

    This kit has been determined to be optimal for transfecting following cell types:

    • 293-F (FreeStyle), 32D
    • A-20, A2058, A375, A431, A549, AGS, ARPE-19
    • BetaTC/6, BxPC-3
    • C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
    • EL4
    • FDC-P1
    • H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
    • IMR-32
    • J774A.1
    • K-562
    • KG-1
    • L-428, LNCaP
    • MDA-MB-453, MDBK, Molt-4, MV-4-11
    • Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
    • P19, P815, PC-12, PC-3
    • Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
    • S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
    • T2, T84, TF-1
    • U266B1, U-937
    • Vero

    The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

    The kit is available in different variants for either 12 or 24 reactions.

    Benefits

    • High transfection efficiencies in difficult-to-transfect cell lines
    • Cells maintain functionality post transfection
    • Conditions are transferable between 4D-NucleofectorTM System (including the , 96-well ShuttleTM System and 384-well NucleofectorTM System

    Applications

    • Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
    • Low throughput applications

    Content & Storage

    Content

    2.25 mL SF Cell Line NucleofectorTM Solution
    0.5 mL Supplement 1
    50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
    24 x 100 µL NucleocuvetteTM Vessel
    24 x Single use pipettes

    lonza Amaxa Nucleofector Kit

    Amaxa Nucleofector 技术是Lonza(原Amaxa)公司的专利创新技术，它综合应用传统的电穿孔技术及细胞特异性细胞核转染液，通过调整优化的电转参数（内存转染程序），直接把外源基因导入原代细胞及细胞系的细胞浆和细胞核中，Nucleofector转染效率最高可达99%。

    Nucleofector技术由Nucleofector仪器、Lonza Amaxa 细胞核转染试剂盒及Lonza Amaxa 细胞核转染试剂盒操作手册组成。

    Lonza Amaxa 细胞核转染试剂盒产品目录：

    货号 分类 UOM 产品描述
    VHNP-1001 Amaxa NF reag PC 96-well Nucleocuvette™ Plate
    V4YP-2A24 Amaxa NF reag EA AD2 4D-Nucleofector™ Y Kit
    V4YP-9A48 Amaxa NF reag EA Primary Cell Opt. 4D-Nucleofector™ Y Kit
    V4XC-3012 Amaxa NF reag KT SG Cell Line 4D X Kit L (12 RCT)
    V4YP-1A24 Amaxa NF reag EA AD1 4D-Nucleofector™ Y Kit
    VAPA-1010 Amaxa NF reag PC 10 RCT Mouse B Cell Kit
    V4XP-5012 Amaxa NF reag KT P5 Primary Cell 4D X Kit L (12 RCT)
    VAMI-1021 Amaxa NF reag PC 10 RCT Basic Parasite Kit 2
    V4XC-2012 Amaxa NF reag KT SF Cell Line 4D X Kit L (12 RCT)
    V4XP-2012 Amaxa NF reag KT P2 Primary Cell 4D X Kit L (12 RCT)
    V4XP-4012 Amaxa NF reag KT P4 Primary Cell 4D X Kit L (12 RCT)
    V4XP-1012 Amaxa NF reag KT P1 Primary Cell 4D X Kit L (12 RCT)
    V4XC-1012 Amaxa NF reag KT SE Cell Line 4D X Kit L (12 RCT)
    VAPA-1005 Amaxa NF reag PC 10 RCT Human NK Cell Kit
    VAPC-1001 Amaxa NF reag PC 10 RCT Human AoSMC Kit
    VAPD-1003 Amaxa NF reag PC 10 RCT NHEM-Neo Kit
    VAPF-1001 Amaxa NF reag PC 10 RCT Human Chondrocyte Kit
    VAPD-1002 Amaxa NF reag PC 10 RCT Human Keratinocyte Kit
    VAPE-1002 Amaxa NF reag PC 10 RCT Rat Cardiomyocyte Neo Kit
    V4XP-3012 Amaxa NF reag KT P3 Primary Cell 4D X Kit L (12 RCT)
    VAMI-1011 Amaxa NF reag PC 10 RCT Basic Parasite Kit 1
    V4XP-2024 Amaxa NF reag KT P2 Primary Cell 4D X Kit L (24 RCT)
    V4XP-5024 Amaxa NF reag KT P5 Primary Cell 4D X Kit L (24 RCT)
    V4XP-4024 Amaxa NF reag KT P4 Primary Cell 4D X Kit L (24 RCT)
    V4XP-1024 Amaxa NF reag KT P1 Primary Cell 4D X Kit L (24 RCT)
    VAPB-1002 Amaxa NF reag PC 10 RCT HUVEC Kit
    V4XC-3024 Amaxa NF reag KT SG Cell Line 4D X Kit L (24 RCT)
    V4XC-1024 Amaxa NF reag KT SE Cell Line 4D X Kit L (24 RCT)
    VAPA-1008 Amaxa NF reag PC 10 RCT Human Macrophage Kit
    VAPA-1009 Amaxa NF reag PC 10 RCT Mouse Macrophage Kit
    V4XP-3024 Amaxa NF reag KT P3 Primary Cell 4D X Kit L (24 RCT)
    V4XC-2024 Amaxa NF reag KT SF Cell Line 4D X Kit L (24 RCT)
    VECA-1001 Amaxa NF reag PC CLB-Transfection™ Kit
    V5SP-9001 Amaxa NF reag PC Primary Cell Optimiz. 384-well Kit (1PT)
    V4XP-1A32 Amaxa NF reag PC Basic Neuron 4D X AD Kit (32 RCT)
    VACA-1004 Amaxa NF reag PC 10 RCT Cell Line Kit C
    V5SC-9001 Amaxa NF reag PC Cell Line Optimization 384-well Kit
    VMI-1001 Amaxa NF reag PC Basic Parasite Nucleofector® Starter Kit
    VAPA-1011 Amaxa NF reag PC 10 RCT Mouse Dendritic Cell Kit
    VAPL-1004 Amaxa NF reag PC 10 RCT Mouse/Rat Hepatocyte Kit
    VAPH-5022 Amaxa NF reag PC 10 RCT Human Stem Cell Kit 2
    VAPD-1005 Amaxa NF reag PC 10 RCT MEF 2 Kit
    VAPE-1001 Amaxa NF reag PC 10 RCT Human MSC Kit
    VAPI-1006 Amaxa NF reag PC 10 RCT Basic Glial Cells Kit
    V4XP-3032 Amaxa NF reag KT P3 Primary Cell 4D X Kit S (32 RCT)
    VAPH-5012 Amaxa NF reag PC 10 RCT Human Stem Cell Kit 1
    VAPA-1001 Amaxa NF reag PC 10 RCT Human B Cell Kit
    VAPA-1004 Amaxa NF reag PC 10 RCT Human Dendritic Cell Kit
    VAPD-1004 Amaxa NF reag PC 10 RCT MEF 1 Kit
    VAPG-1004 Amaxa NF reag PC 10 RCT Mouse Neural Stem Cell Kit
    VAPI-1003 Amaxa NF reag PC 10 RCT Basic Primary Neurons Kit
    VAPI-1004 Amaxa NF reag PC 10 RCT Basic SMC Kit
    VVPA-1006 Amaxa NF reag KT 100 RCT Mouse T Cell Kit
    VAPD-1001 Amaxa NF reag PC 10 RCT NHDF Kit
    VAPI-1002 Amaxa NF reag PC 10 RCT Basic Fibroblasts Kit
    VAPA-1002 Amaxa NF reag PC 10 RCT Human T Cell Kit
    VAPH-1001 Amaxa NF reag PC 10 RCT Mouse ES Cell Kit
    VAPI-1001 Amaxa NF reag PC 10 RCT Basic Endothelial Cells Kit
    VAPA-1003 Amaxa NF reag PC 10 RCT Human CD34 Cell Kit
    VAPG-1001 Amaxa NF reag PC 10 RCT Mouse Neuron Kit
    VAPG-1003 Amaxa NF reag PC 10 RCT Rat Neuron Kit
    VAPI-1005 Amaxa NF reag PC 10 RCT Basic Epithelial Cells Kit
    VVPB-1001 Amaxa NF reag KT 100 RCT HCAEC Kit
    VVPB-1003 Amaxa NF reag KT 100 RCT HMVEC-L Kit
    VVPB-1492 Amaxa NF reag KT 100 RCT HUVEC OLD Kit
    VCO-1001N Amaxa NF reag PC Cell Line Optimization Nucleofector® Kit
    VACA-1005 Amaxa NF reag PC 10 RCT Cell Line Kit L
    VVPA-1010 Amaxa NF reag KT 100 RCT Mouse B Cell Kit
    VVPH-1001 Amaxa NF reag KT 100 RCT Mouse ES Cell Kit
    VACA-1001 Amaxa NF reag PC 10 RCT Cell Line Kit R
    VACA-1002 Amaxa NF reag PC 10 RCT Cell Line Kit T
    VPH-5002 Amaxa NF reag PC Human StemCell Nucleofector® Starter Kit
    VPD-1006 Amaxa NF reag PC MEF Starter Nucleofector® Kit
    VPA-1006 Amaxa NF reag PC Mouse T Cell Nucleofector® Kit
    VVPK-1002 Amaxa NF reag KT 100 RCT HMEC Kit
    VVPA-1008 Amaxa NF reag KT 100 RCT Human Macrophage Kit
    VVPA-1009 Amaxa NF reag KT 100 RCT Mouse Macrophage Kit
    VACA-1003 Amaxa NF reag PC 10 RCT Cell Line Kit V
    VVMI-1021 Amaxa NF reag KT 100 RCT Basic Parasite Kit 2
    VVPH-5012 Amaxa NF reag KT 100 RCT Human Stem Cell Kit 1
    VVPH-5022 Amaxa NF reag KT 100 RCT Human Stem Cell Kit 2
    VVPA-1005 Amaxa NF reag KT 100 RCT Human NK Cell Kit
    VVPF-1001 Amaxa NF reag KT 100 RCT Human Chondrocyte Kit
    VPB-1001 Amaxa NF reag PC HCAEC Nucleofector® Kit
    VPB-1003 Amaxa NF reag PC HMVEC-L Nucleofector® Kit
    VVPE-1002 Amaxa NF reag KT 100 RCT Rat Cardiomyocyte Neo Kit
    VVPG-1002 Amaxa NF reag KT 100 RCT Chicken Neuron Kit
    VVPB-1002 Amaxa NF reag KT 100 RCT HUVEC Kit
    VVPC-1001 Amaxa NF reag KT 100 RCT Human AoSMC Kit
    VVPD-1003 Amaxa NF reag KT 100 RCT NHEM-Neo Kit
    VVPL-1004 Amaxa NF reag KT 100 RCT Mouse/Rat Hepatocyte Kit
    VPB-1492 Amaxa NF reag PC HUVEC OLD Nucleofector® Kit
    VVPI-1003 Amaxa NF reag KT 100 RCT Basic Primary Neurons Kit
    VVPA-1011 Amaxa NF reag KT 100 RCT Mouse Dendritic Cell Kit
    VVCA-1004 Amaxa NF reag KT 100 RCT Cell Line Kit C
    VVSI-1003 Amaxa NF reag KT 100 RCT Basic Neuron SCN Kit
    VVPA-1001 Amaxa NF reag KT 100 RCT Human B Cell Kit
    VVPA-1003 Amaxa NF reag KT 100 RCT Human CD34 Cell Kit
    VVPA-1004 Amaxa NF reag KT 100 RCT Human Dendritic Cell Kit
    VVPA-1007 Amaxa NF reag KT 100 RCT Human Monocyte Kit
    VVPD-1002 Amaxa NF reag KT 100 RCT Human Keratinocyte Kit
    VVPD-1004 Amaxa NF reag KT 100 RCT MEF 1 Kit
    VVPD-1005 Amaxa NF reag KT 100 RCT MEF 2 Kit
    VVPE-1001 Amaxa NF reag KT 100 RCT Human MSC Kit
    VVPG-1005 Amaxa NF reag KT 100 RCT Rat Neural Stem Cell Kit
    VVPI-1001 Amaxa NF reag KT 100 RCT Basic Endothelial Cells Kit
    VVPI-1005 Amaxa NF reag KT 100 RCT Basic Epithelial Cells Kit
    VVPI-1006 Amaxa NF reag KT 100 RCT Basic Glial Cells Kit
    VVMI-1011 Amaxa NF reag KT 100 RCT Basic Parasite Kit 1
    VVPI-1004 Amaxa NF reag KT 100 RCT Basic SMC Kit
    VVPI-1002 Amaxa NF reag KT 100 RCT Basic Fibroblasts Kit
    VVPG-1004 Amaxa NF reag KT 100 RCT Mouse Neural Stem Cell Kit
    VVPG-1001 Amaxa NF reag KT 100 RCT Mouse Neuron Kit
    V4XP-5032 Amaxa NF reag KT P5 Primary Cell 4D X Kit S (32 RCT)
    VPA-1010 Amaxa NF reag PC Mouse B Cell Nucleofector® Kit
    VVPD-1001 Amaxa NF reag KT 100 RCT NHDF Kit
    VVCA-1001 Amaxa NF reag KT 100 RCT Cell Line Kit R
    VVCA-1005 Amaxa NF reag KT 100 RCT Cell Line Kit L
    VVCA-1002 Amaxa NF reag KT 100 RCT Cell Line Kit T
    VVPA-1002 Amaxa NF reag KT 100 RCT Human T Cell Kit
    VVPG-1003 Amaxa NF reag KT 100 RCT Rat Neuron Kit
    VVCA-1003 Amaxa NF reag KT 100 RCT Cell Line Kit V
    VPK-1002 Amaxa NF reag PC HMEC Nucleofector® Kit
    VPA-1008 Amaxa NF reag PC Human Macrophage Nucleofector® Kit
    VPA-1009 Amaxa NF reag PC Mouse Macrophage Nucleofector® Kit
    VMI-1021 Amaxa NF reag PC Basic Parasite Nucleofector® Kit 2
    VPH-5012 Amaxa NF reag PC Human Stem Cell Nucleofector® Kit 1
    VPH-5022 Amaxa NF reag PC Human Stem Cell Nucleofector® Kit 2
    VPA-1005 Amaxa NF reag PC Human NK Cell Nucleofector® Kit
    VPF-1001 Amaxa NF reag PC Human Chondrocyte Nucleofector® Kit
    V4XP-2032 Amaxa NF reag KT P2 Primary Cell 4D X Kit S (32 RCT)
    VPE-1002 Amaxa NF reag PC Rat Cardiomyocyte Neo Nucleofector® Kit
    VPG-1002 Amaxa NF reag PC Chicken Neuron Nucleofector® Kit
    VPB-1002 Amaxa NF reag PC HUVEC Nucleofector® Kit
    V4XP-4032 Amaxa NF reag KT P4 Primary Cell 4D X Kit S (32 RCT)
    V4SP-2096 Amaxa NF reag KT P2 Primary Cell 96-well Kit (96 RCT)
    VPC-1001 Amaxa NF reag PC Human AoSMC Nucleofector® Kit
    VPD-1003 Amaxa NF reag PC NHEM-Neo Nucleofector® Kit
    VPL-1004 Amaxa NF reag PC Mouse/Rat Hepatocyte Nucleofector™ Kit
    VPI-1003 Amaxa NF reag PC Basic Primary Neurons Nucleofector® Kit
    VPA-1011 Amaxa NF reag PC Mouse Dendritic Cell Nucleofector® Kit
    VCA-1004 Amaxa NF reag PC Cell Line Nucleofector® Kit C
    VSPI-1003 Amaxa NF reag PC Basic Neuron SCN Nucleofector® Kit
    VPA-1001 Amaxa NF reag PC Human B Cell Nucleofector® Kit
    VPA-1003 Amaxa NF reag PC Human CD34 Cell Nucleofector® Kit
    VPA-1004 Amaxa NF reag PC Human Dendritic Cell Nucleofector® Kit
    VPA-1007 Amaxa NF reag PC Human Monocyte Nucleofector® Kit
    VPD-1002 Amaxa NF reag PC Human Keratinocyte Nucleofector® Kit
    VPD-1004 Amaxa NF reag PC MEF 1 Nucleofector® Kit
    VPD-1005 Amaxa NF reag PC MEF 2 Nucleofector® Kit
    VPE-1001 Amaxa NF reag PC Human MSC Nucleofector® Kit
    VPG-1005 Amaxa NF reag PC Rat Neural Stem Cell Nucleofector® Kit
    VPH-1001 Amaxa NF reag PC Mouse ES Cell Nucleofector® Kit
    VPI-1001 Amaxa NF reag PC Basic Endothel. Cells Nucleofector® Kit
    VPI-1005 Amaxa NF reag PC Basic Epithelial Cells Nucleofector® Kit
    VPI-1006 Amaxa NF reag PC Basic Glial Cells Nucleofector™ Kit
    V4XP-1032 Amaxa NF reag KT P1 Primary Cell 4D X Kit S (32 RCT)
    VMI-1011 Amaxa NF reag PC Basic Parasite Nucleofector® Kit 1
    VPI-1004 Amaxa NF reag PC Basic SMC Nucleofector® Kit
    V4SP-5096 Amaxa NF reag KT P5 Primary Cell 96-well Kit (96 RCT)
    V4XC-3032 Amaxa NF reag KT SG Cell Line 4D X Kit S (32 RCT)
    V4XC-1032 Amaxa NF reag KT SE Cell Line 4D X Kit S (32 RCT)
    V4SP-4096 Amaxa NF reag KT P4 Primary Cell 96-well Kit (96 RCT)
    V4SP-1096 Amaxa NF reag KT P1 Primary Cell 96-well Kit (96 RCT)
    VPI-1002 Amaxa NF reag PC Basic Fibroblasts Nucleofector® Kit
    VPG-1004 Amaxa NF reag PC Mouse Neural Stem Cell Nucleofector® Kit
    VPG-1001 Amaxa NF reag PC Mouse Neuron Nucleofector® Kit
    VPD-1001 Amaxa NF reag PC NHDF Nucleofector® Kit
    VCA-1001 Amaxa NF reag PC Cell Line Nucleofector® Kit R
    VCA-1005 Amaxa NF reag PC Cell Line Nucleofector® Kit L
    VCA-1002 Amaxa NF reag PC Cell Line Nucleofector® Kit T
    VPA-1002 Amaxa NF reag PC Human T Cell Nucleofector® Kit
    VPG-1003 Amaxa NF reag PC Rat Neuron Nucleofector® Kit
    V4XP-9096 Amaxa NF reag PC Primary Cell Optimiz. 4D X Kit (96 RCT)
    V4SC-3096 Amaxa NF reag KT SG Cell Line 96-well Kit (96 RCT)
    V4SC-1096 Amaxa NF reag KT SE Cell Line 96-well Kit (96 RCT)
    V4XC-2032 Amaxa NF reag KT SF Cell Line 4D X Kit S (32 RCT)
    VCA-1003 Amaxa NF reag PC Cell Line Nucleofector® Kit V
    V4SP-3096 Amaxa NF reag KT P3 Primary Cell 96-well Kit (96 RCT)
    V4SC-2096 Amaxa NF reag KT SF Cell Line 96-well Kit (96 RCT)
    V4XC-9064 Amaxa NF reag PC Cell Line Optimization 4D X Kit (64 RCT)
    V4SC-9096 Amaxa NF reag PC Cell Line Optimiz. 96-well Kit (96 RCT)
    VS1-00500P Amaxa NF reag PC Nucleofector™ PLUS-1 Supplement, 500µl
    VGA-1003 Amaxa NF reag PC cGMP Cell Line Nucleofector® Kit V
    VSOP-2096 Amaxa NF reag KT P2 Primary Cell solution (1x 96)
    VSOP-5096 Amaxa NF reag KT P5 Primary Cell solution (1x 96)
    VSOP-4096 Amaxa NF reag KT P4 Primary Cell solution (1x 96)
    VSOP-1096 Amaxa NF reag KT P1 Primary Cell solution (1x 96)
    VSOC-3096 Amaxa NF reag KT SG Cell Line Solution (1x 96)
    VSOC-1096 Amaxa NF reag KT SE Cell Line Solution (1x 96)
    VSOP-3096 Amaxa NF reag KT P3 Primary Cell solution (1x 96)
    VSOC-2096 Amaxa NF reag KT SF Cell Line Solution (1x 96)
    V4XCG-2024 Amaxa NF reag PC cGMP Solution SF 4D-Nucleofector™ Kit
    V4XPG-3024 Amaxa NF reag PC cGMP Solution P3 4D-Nucleofector™ Kit
    VASI-1003 Amaxa NF reag PC 10 RCT Basic Neuron SCN Kit
    VS4-00500P Amaxa NF reag PC Nucleofector™ PLUS-4 Supplement, 500µl
    VAPB-1001 Amaxa NF reag PC 10 RCT HCAEC Kit
    VAPB-1003 Amaxa NF reag PC 10 RCT HMVEC-L Kit
    VS3-00150P Amaxa NF reag PC Nucleofector™ PLUS-3 Supplement, 150µl
    VAPK-1002 Amaxa NF reag PC 10 RCT HMEC Kit
    VAPG-1002 Amaxa NF reag PC 10 RCT Chicken Neuron Kit
    VS3-00500P Amaxa NF reag PC Nucleofector™ PLUS-3 Supplement, 500µl
    VAPG-1005 Amaxa NF reag PC 10 RCT Rat Neural Stem Cell Kit
    VS2-00500P Amaxa NF reag PC Nucleofector™ PLUS-2 Supplement, 500µl
    VS1-00150P Amaxa NF reag PC Nucleofector™ PLUS-1 Supplement, 150µl
    VIPI-1003 Amaxa NF reag PC Basic Neuron 96-well AD Kit
    VGA-1005 Amaxa NF reag PC cGMP Cell Line Nucleofector® Kit L

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