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- Thermo Scientific™
- R0531
- 2025年10月02日
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- 详细信息
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- 文献和实验
- 技术资料
- 库存：
10
- 英文名：
TurboFect Transfection Reagent
- 供应商：
上海觅拓生物
- 规格：
1ml
描述
Thermo Scientific TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.Highlights
• Excellent transfection efficiency in the presence or absence of serum
• Ready-to-use reagent – no need to reconstitute, dilute, or manipulate
• Simple protocol
High transfection efficiency of plasmid DNA into a wide variety of cell types
• Adherent and suspension cells
• Differentiated and undifferentiated cells
Review our experimental considerations to improve your plasmid transfection results.
规格
| Cell Type: | Endothelial Cells, Epithelial Cells, Established Cell Lines, Fibroblasts, Hepatocytes, Kidney, Macrophages, Myeloma Cells, Primary Cells |
|---|---|
| Sample Type (Specific): | Plasmid DNA |
| Classification: | Chemically Defined |
| Transfection Technique: | Chemical Transfection |
| Product Line: | TurboFect |
| Product Size: | 1 mL |
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文献和实验本制品是一种快速方便的总RNA提取试剂，可以从动物组织、植物材料、各种微生物、培养细胞等中提取总RNA。样品在RNAiso Reagent中能够充分被裂解，在加入氯仿离心后，溶液会形成上清层、中间层和有机层（下层)，RNA分布在上清层中，收集上清层后，经异丙醇沉淀便可以回收得到总RNA。RNAiso Reagent具有很强的广谱性，可以适用于各种样品的总RNA提取，提取过程方便快速，整个操作在一小时内便可以完成。经本制品提取的总RNA纯度高，基本不含蛋白质及基因组 DNA，提取的RNA可以直接
Cationic Liposome-Mediated Transfection with Lipofectin Reagent
Since their original description, liposomes have been discussed as vehicles that could be used as carriers of pharmaceutically active agents (1 ), and their potential for use as carriers of genetic information has been examined
Procedure for Transfection of Mammalian Cells
(containing nonessential amino acids). Solution B: For each transfection, dilute 12 μl LIPOFECTAMINE Reagent in 375 μl serum-free IMDM. Combine the two solutions, mix gently, and incubate at room temperature for 15-45 min. The solution may appear
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