- 询价
- Thermo scientific -fermentas
- R0541
- 2025年10月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
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大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| R0541 | 120 µl (for 20 transfections using 50 μg DNA) | R0541 |
- Features
-
- 无可检测的炎症反应。
- 通过多种方式转染DNA。
- 实验结果可重复。
- 经小鼠和大鼠功能测试。
- Applications
-
- DNA转染。
- siRNA-DNA共转染。
50 µg质粒DNA与TurboFect™ in vivo Transfection Reagent混合后经静脉内注射转入雌性BALB/c小鼠。在不同时间提取血清样本，采用ELISA方法检测细胞因子TNFα浓度。
50 µg编码萤火虫荧光素酶基因的质粒DNA与体内转染试剂形成复合物，通过静脉内注射导入雌性BALB/c小鼠。转染方法根据生产商的使用推荐。转染24小时后，检测不同器官内的萤火虫荧光素酶表达。
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- 作者
- 内容
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文献和实验transfection procedures/reagents, DEAE dextran, calcium phosphate and Effectene™. Aims You will work individually, to perform your transfections Ð two people will work at the same time in the tissue culture facilities in the Wolfson building.
Transfection of Primary Cultures of Rat Hepatocytes
Five different transfection reagents—calcium phosphate, TransFast™ Transfection Reagent, Superfect™ Transfection Reagent, Effectene™ Transfection Reagent, and Tfx™-20—were compared for their ability to effectively transfect primary cultures
Transient Transfection and Adrenergic Receptor Promoter Analysis
well for transfection of β 1 -AR gene fragments into adherent cell lines, but is much less effective for transfection of DNA into primary cultures. As noted by other investigators ( 5 ), we have applied FuGENE™ 6 transfection reagent, a nonliposomal lipid compound
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