- ￥29687
- Polyplus Transfection
- 101000001
- 2025年10月03日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海觅拓
- 规格：
5 x (1.5 ml of transfection reagent) + 10 x (60 ml of jetPRIME buffer)
Polyplus Transfection 提供全新的阳离子聚合物转染试剂，其产品适用于体内和体外转染，包括DNA、 siRNA、寡核苷酸及蛋白转染，可进行瞬时和稳定的转染,公司拥有ISO9001:2000证书。
jetPRIME®
- High DNA transfection efficiency
- Low amounts of nucleic acid
- Superior cell viability
- ONE reagent for DNA and/or siRNA transfections
- Cost-effective
| Reagent | jetPRIME® |
|---|---|
| Molecule delivered | DNA, siRNA, DNA & siRNA |
| Applications | Plasmid transfection |
| Cell types | Adherent cell lines grown in presence of serum |
| Number of transfections | 1.5 ml of jetPRIME® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | Store jetPRIME® at 5 °C ± 3°C. |
| Provided with | jetPRIME® buffer |
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文献和实验Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
E-Z 96® M13 Isolation Vacuum Manifold Protocol
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (96%-100%) ethanol 实验设备 1. Microcentrifuge capable of at least 10,000 x g 2. Sterile 15 mL centrifuge tubes 3. Sterile
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
and then at 280 nm. The DNA concentration is calculated as follows: DNA concentration = Absorbance260 × 50 × (Dilution Factor) :g/ml Although the binding capacity of HiBind® DNA Column is around 35 ug, the yield of the yeast plasmid depends
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