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- Roche
- 11434993103
- 2025年08月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
嵘崴达
- 英文名：
GlDH, Modified from Beef Liver
- 库存：
需确认
- 保存条件：
见说明书
- 保质期：
见产品外包装
from beef liver, chemically modified, lyophilizate 11 434 993 103
Dehydrogenase that catalyzes the conversion of glutamate to α-ketoglutarate.
Application
Use modified Glutamate Dehydrogenase in diagnostic tests for the determi-
nation of ammonia, urea, L-glutamate, glutamate pyruvate transaminase and
leucine aminopeptidase.
Benefits
Take advantage of the enhanced liquid stability. ■
Rely on the proven diagnostic quality of this product. ■
EC 1.4.1.3
Properties
Nomenclature: L-glutamate:NAD(P) + oxidoreductase (deaminating)
Molecular weight: ~2 200 kD for the associated enzyme with 8 subunits; 280
kD for one subunit.
Michaelis constants (Tris buffer, pH 8.0, +23°C):
L-glutamate: 1.8 x 10 -3 mol/l
NADP: 4.7 x 10 -5 mol/l
α-ketoglutarate: 7.0 x 10 -4 mol/l
NH 4 + : 3.2 x 10 -3 mol/l
NADPH: 2.6 x 10 -5 mol/l
Km values for NAD or NADH are difficult to obtain due to their inhibitory ac-
tion.
Inhibitors: 4-chloromercuribenzoate, Na 2 S, diethyldithiocarbamate,
1,10-phenanthroline,8-hydroxyquinoline, NaN 3 , thyroxine, heparin, sulfonylcarbamides, Cu 2+ , Hg 2+ ,
Ag 2+ , Fe 3+ , Zn 2+ , K + , PO 4 2- , NO 3 -
Activators: Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
pH optimum: 8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.5-6.5 (see figure)
Thermal stability: Up to +60°C (see figure)
Specificity: The oxidation of L-glutamate is stimulated by ADP and inhibited
by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine,
valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and
inhibited by ADP.
Remarks:
- Glutamate dehydrogenase suspension or solution can be dialyzed against
phosphate buffer, 10 mmol/l. Glutamate dehydrogenase molecules have
the tendency to associate in some test formulations, modified Glutamate
dehydrogenase minimizes this effect.
-The modified enzyme is especially suited for liquid stable applications with
extended shelf life requirements.
Specification
Appearance: White lyophilizate, stabilized with RPLA 4 and ADP
Solubility: Clear, slightly opalescent solution in water (c=40 mg/ml)
Activity (+25°C, α-oxoglutarat): ≥7 U/mg lyophilizate
Contaminants (expressed as percentage of Glutamate Dehydrogenase
activity):
Alcohol dehydrogenase: ≤0.005
Lactate dehydrogenase: ≤0.005
Malate dehydrogenase: ≤0.005
NH 4 : ≤0.16 μmol/KU GIutamate Dehydrogenase
K (flame photometric): ≤0.1 μmol/KU Glutamate Dehydrogenase
Na (flame photometric): ≤2.0 μmol/KU Glutamate Dehydrogenase
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months. Store dry.
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文献和实验数据。 小师妹：原来这个意思，和以前筛菌一样，快了多了就叫高通量筛选。那不是人人都能做？ 霸霸：错，那可是有技术含量的。目前主要的平台有罗氏的 454 测序仪、Illumina 的 Solexa 基因组分析仪、ABI 的 SOLiD 测序仪。 接下去就给大家具体介绍这三个测序仪都使用的是啥技术？有啥共同点呢？ 454：焦磷酸测序 待测 DNA 样品被打断成 300-800 bp 的片段后，3' 和 5'端分别加上接头，这些接头会使 DNA 片段结合到微珠
，罗氏用的磁微粒子分离是国内厂家可以采用的，但定货量上不去的话成本很高，与beckma,bayer,罗氏的全球市场比起来，单一个中国市场（而且还只是中国市场的一部分）的用量实在是很难拿到很好的价格。如果用板式的方法来进行分离，那也就只能在国内的二三级市场混了，而且还朝不保夕。目前一个解决办法是自己开发合成，而这又受到技术与原料方面的限制，特别是原料，我曾经找过一些国内的大学与研究院所进行过合作，但结果很一般（不说细节，结果如此。），纠其根本，就是技术与原料的问题，特别是原料，主要的原料进口的太贵
罗氏推出最新款实时荧光PCR仪LightCycler® 96
研究领域的产品，并秉承不断投入创新的精神，现已构建了一套从基因组学，到细胞组学及蛋白组学方面的高效、完整研究解决方案。 与此同时，作为世界领先的制药和体外试剂的生产厂商之一，罗氏诊断应用科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断应用科学部为客户进行量身定制产品，满足客户个性化需求。
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