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- roche
- 罗氏10745731001
- 2025年11月07日
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- 保质期：
见产品外包装
- 供应商：
嵘崴达
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- 英文名：
β-Galactosidase EIA grade
- 规格：
25mg
β-Galactosidase
EIA grade
别名:
β galactosidase, β-galactosidase
一般描述
The GLB1 (β-Galactosidase) gene is mapped to human chromosome 3p22.3. The encoded protein belongs to glycosyl hydrolase 35 family and is localized to lysosomes.
β-Galactosidase, EIA grade, is a lyophilizate from E. coli overproducer, consisting of enzyme protein, phosphate buffer, and sucrose. Substances which could interfere with the derivatization of NH2 or SH groups (e.g., 2-mercaptoethanol, ammonium salts, primary amines etc.) have been removed.
应用
β-Galactosidase is used for labeling enzyme immunoassay techniques. β-Galactosidase can be coupled to other proteins via its SH-groups. The reconstituted solution can be used directly for conjugation without prior dialysis or gel permeation chromatography.
β-Galactosidase has been used as a standard to determine the absolute quantitation of LacZ protein.
生化/生理作用
β-Galactosidase catalyzes the hydrolysis of terminal β-galactose residue of ganglioside substrates, such as glycoproteins, sphingolipids, and keratan sulfate and other glycoconjugates. This enzyme is associated with the mechanism of cell senescence and carcinogenesis. Mutations in the gene result in gangliosidosis, an autosomal recessive disorder, characterized with defective lysosomal storage due to accumulation of substrates. β-Galactosidase deficiency also causes Morquio B syndrome indicating skeletal abnormalities, short stature and increased excretion of keratan sulfate in urine.
质量
Purity: single peak (HPLC)
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
" moiety (5-bromo-4-chloro-indolyl) in both X-gal and X-Phos is the same and thus once cleavage by the respective enzyme has occurred, the dimerization and oxidation reactions lead to the same halogenated indigo compound. B. Nitroblue tetrazolium (NBT
两个酶：促使乳糖进入细菌的乳糖透过酶(lactose permease)催化乳糖分解第一步的β－半乳糖苷酶(β－galactosidase)(图19-2)。 在环境中没有乳糖或其他β-半乳糖苷时，大肠杆菌合成β-半乳糖苷酶量极少，加入乳糖2－3分钟后，细菌大量合成β-半乳糖苷酶，其量可提高千倍以上，在以乳糖作为唯一碳源时，菌体内的β-半乳糖苷酶量可占到细菌总蛋白量的3%。在上述二阶段生长细菌利用乳糖再次繁殖前，也能测出细菌中β-半乳糖苷酶活性显著增高的过程。这种典型的诱导现象，是研究
A Guide to Yeast Two-Hybrid Experiments（酵母双杂交实验指南）
. The diploid cells can be assayed for reporter gene activity by testing for growth on selective media or β-galactosidase activity (from the lacZ reporter). (B) Screening approaches. Library and high-throughput screens are based on some variation
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