- ￥19902
- roche
- 4710452001
- 2025年10月28日
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- 详细信息
- 技术资料
- 库存：
需确认
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 英文名：
FastStart™ PCR Master
- 保存条件：
−20°C
- 规格：
10 X 5 ML
FastStart ™ PCR启动酶
FastStart™ PCR Master
| packaging | pkg of 2 × 1.25 mL (04710436001) |
| pkg of 8 × 1.25 mL (04710444001) | |
| pkg of 10 × 5 mL (04710452001) |
General description
Hot start polymerase chain reaction (PCR) is effortless using the FastStart™ PCR Master, a ready-to-use, double-concentrated hot start master mix. This master mix contains FastStart Taq DNA Polymerase, Roche high quality PCR-Grade nucleotides and all other reagents required for PCR and two-step reverse transcriptase (RT)-PCR on thermal cycler instruments.
FastStart™ PCR Master accepts modified nucleotides such as Digoxigenin (DIG)-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 μM to the final volume.
Combine this master mix with Roche′s Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step RT-PCR.
Application
FastStart™ PCR Master has been used for the analysis of Nosema sp. DNA by polymerase chain reaction (PCR) method for validating the genomic DNA by PCR , for amplification of RNA by PCR method.
The FastStart™ PCR Master is a convenient alternative to the single reagents for nearly all hot start PCR applications. It can be applied for the amplification of genomic DNA and cDNA targets up to 2 kb long, or longer fragments on plasmid DNA targets, with high specificity, sensitivity, and yield. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. It can be used for:
• Hot Start PCR up to 2 kb
• Hot Start RT-PCR up to 2 kb
• High-throughput PCR
• Direct colony PCR
Features and Benefits
FastStart Master已修饰重组FastStart Taq DNA聚合酶，在低于+ 75°C的温度下无活性。该试剂盒还包含优化的PCR缓冲液和富含 GC 的溶液，用于处理各种模板。
| 4710452001 | FastStart PCR Master 50 ml | 10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume) |
| 11814362001 | PCR Nucleotide Mix (2000 react | 2,000 µl (10 x 200 µl) (for 2,000 reactions of 50 µl final reaction volume) |
| 4738276001 | Expand HiFi PCR System dNTPack | 2,500 U (10 x 250 U) |
| 4673450001 | FastStart TaqMan Probe Master | 2 x 1.25 ml (for 100 reactions of 50 µl final reaction volume) |
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