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- 2025年11月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 英文名：
Restriction Endonuclease Not I
- 库存：
需确认
- 供应商：
嵘崴达
- 保质期：
见产品外包装
- 规格：
1000 UNITS
Not I
from Nocardia otitidis-caviarum
别名:
restriction enzyme
一般描述
Not I recognizes the sequence GC?GG*C*CG°C and generates fragments with 5′-cohesive termini.
Not I belongs to the class of "rare-cutter" enzymes. It is one of the two known enzymes that recognize an octameric sequence comprised solely of G and C residues.
Contents:
- Not I
- SuRE/Cut Buffer H (10x)
特异性
Recognition sites: GCGG*C*CG °C
GCGG*C*CG °C
Restriction site: GC↓GG*C*CG °C
GC↓GG*C*CG °C
Heat inactivation: Not I can be heat inactivated by incubation at 65 °C for 15 minutes (up to 100 U/μg DNA).
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主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
R&D试剂，周一定货，3-4周到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
罗氏原料，请来电来邮咨询。
OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。
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文献和实验Restriction Endonuclease Digestion of DNA
The ability to cleave DNA at specific sites is one of the cornerstones of today’s methods of DNA manipulation. Restriction endonucleases are bacterial enzymes that cleave duplex DNA at specific target sequences with the production of defined
Isolation and Characterization of an Unknown Restriction Endonuclease
contain at least one restriction endonuclease (2 ), and therefore the probability of encountering new ones is relatively high. Indeed, many new enzymes have been “discovered” in contaminated bacterial cultures. The presence of three restriction activities
Restriction Endonuclease Digestion and Agarose Gel Electrophoresis of DNA
. These enzymes are known as Type II restriction endonucleases, and they allow us to cut DNA molecules in a reproducible fashion. This precision cutting is essential for many of the procedures of genetic manipulation. Circular plasmid molecules must be cut
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