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QAbio
上海钰博生物科技有限公司
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国别:美国QAbio
QAbio Product categories
Endoglycosidases
Exoglycosidases
Deglycosylation Kits
Sialic Acid Products
Glycan Labeling
Capture and Cleanup
Glycan Standards
Columns and Buffers
New Products
Glycoprofiling is used by the biopharmaceutical industry to analyze the glycans found on glycoproteins being developed as therapeutics. It is also a key assay for the lot release characterization of manufactured glycoproteins both before and after approval.
The process can be broken down as follows:
Typically, PNGase F is used to release N-linked glycans. The enzyme works quickly and effectively, cleaving the glycans in native conditions in most cases. The Endo F family of enzymes are also commonly used for glycan release. These three enzymes vary in specificity to the multi-antennary structures found on N-linked glycans. They also differ from PNGase F in that they leave the penultimate glycan, a charged N-acetyl-glucosamine, attached to the protein. Both PNGase F and the Endo F family of enzymes leave the glycan with a free reducing terminii suitable for fluorescent labelling.
For O-linked glycan release, the Ludger Liberate Orela Kit allows the release of glycans that have free reducing terminii suitable for fluorescent labelling. The kit is straightforward and contains a simple method with no special handling techniques as required by the alternative method, hydrazinolysis.
A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents.
Glycans are then typically labeled with a reporter molecule based upon the separation/detection systems being used for the analysis. Ludger has developed a variety of robust and reliable glycan labeling kits.
Fluorescent molecules 2-AB and 2-AA have been used for glycan analysis in HPLC assays for many years and are considered a gold standard within the industry.
Permethylation labeling kits are available for mass spectrometry and APTS have been developed for capillary electrophoresis.
Procainamide labelling permits glycan identification by either mass spectrometry or UHPLC, and because of its improved ionisation efficiency it can permit identification of minor glycans (>1% relative peak area) by ESI-MS.
We supply a full line of Ludger HPLC columns and buffers developed specifically for glycan analysis.
QA-Bio also supplies a wide variety of qualitative and quantitative glycan standards and glycoprotein standards to be used during glycoprofiling. All are produced at the highest quality and purity levels.
Sialic Acid Analysis
The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids.
The kit includes all the reagents to release sialic acids from your glycoprotein and label these with DMB.
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LT-KDMB-A1
Developed for the analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.
Dimensions: 2.1mm x 100mm
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LS-UR2-2.1x100
The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).
View product documentation as PDF
Part Number: KE-SIALIQ
Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase
View product documentation as PDF
Part Number: E-ALD01
This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.
View product documentation as PDF
Part Number: E-CMP01
recombinant from A. ureafaciens
View product documentation as PDF
Part Number: E-S001
recombinant from C. perfringens
View product documentation as PDF
Part Number: E-S005
recombinant from Streptococcus pneumoniae in E. Coli
View product documentation as PDF
Part Number: E-S007
Monosaccharide Analysis
This kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LT-MONO-96
View product documentation as PDF
View Certificate of Analysis as PDF
Dimensions: 2.1mm x 50mm
Part Number: LS-UR2-2.1x50
Mix of six monosaccharide quantitative standards
View product documentation as PDF
Part Number: CM-MONOMIX-10
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