to 50.
If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step.
Rabbits:
Rabbits are immunized essentially as described above. Injections:
First in Freund''s
. The choice between using polyclonal and/or monoclonal antibodies will govern the basic approach. Each approach will present its own advantages/disadvantages including cost, ability to produce a high yield, quality, and a need for standardization. The context
Several antireceptor monoclonal antibodies (MAbs) have been described to compete with ligands for receptor binding. The possibility that structures derived from the hypervariable or complementarity determining regions (CDR) of such antibodies