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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Eco147I (StuI) (10 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实在有限公司
- 保存条件：
-20℃
- 规格：
5000units
Eco147I (StuI) (10 U/µL)
Enzyme : Eco147I (StuI)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer B
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : Not CpG methylation-sensitive, Not dam methylation-sensitive, dcm methylation-sensitive
5' A G G ↓ C C T 3'
3' T C C ↑ G G A 5'
Thermo Scientific Eco147I (StuI) restriction enzyme recognizes AGG^CCT sites and cuts best at 37°C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: Eco147I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.
For methylation sensitivity, refer to product specifications.
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文献和实验冻融 . 7. 不能检测含NaN3的样品 ，因NaN3 抑制辣根过氧化物酶的（ HRP ）活性。 操作步骤 1. 标准品的稀释与加样：在酶标包被板上设标准品孔10 孔，在第一、第二孔中分别加标准品 100 μ l，然后在第一、第二孔中加标准品稀释液 50 μ l，混匀；然后从第一孔、第二孔中各取 100 μ l分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50 μ l，混匀；然后在第三孔和第四孔中先各取 50 μ l弃掉，再各取 50 μ l分别加到第五
冻融 . 7. 不能检测含NaN3的样品 ，因NaN3 抑制辣根过氧化物酶的（ HRP ）活性。 操作步骤 1. 标准品的稀释与加样：在酶标包被板上设标准品孔10 孔，在第一、第二孔中分别加标准品 100 μ l，然后在第一、第二孔中加标准品稀释液 50 μ l，混匀；然后从第一孔、第二孔中各取 100 μ l分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50 μ l，混匀；然后在第三孔和第四孔中先各取 50 μ l弃掉，再各取 50 μ l分别加到第五
NaCl Conc. Stock Volume 10 mM 1 M Tris pH 7.5 100 µl 15 mM 5 M NaCl 30 µl H2O to 10 ml Store at 4 C. 10X TBE Buffer (pH 8) 1X 1L 10X 4L 10X 89 mM Tris-Base 108 g 432 g 89 mM Boric acid 55 g 220 g 2 mM Na2EDTA 9.3 g 37.2 g
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