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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Psp5II (PpuMI) (10 U/µL)
- 保质期：
1年
- 供应商：
上海沪震实在有限公司
- 保存条件：
-20℃
- 规格：
500units
Psp5II (PpuMI) (10 U/µL)
Enzyme : Psp5II (PpuMI)保存温度 : -20℃
货期 : 2-3天
Compatible Buffer : 10x Buffer G
Optimal Reaction Temperature : 37° C
Sensitive to Heat Inactivation: : Yes
Methylation Sensitivity : Not CpG methylation-sensitive, Not dam methylation-sensitive, dcm methylation-sensitive
5' R G ↓ G W C C Y 3'
3' Y C C W G ↑ G R 5'
Thermo Scientific Psp5II (PpuMI) restriction enzyme recognizes RG^GWCCY sites and cuts best at 37°C in G buffer (Isoschizomers: PpuMI, PspPPI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
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文献和实验Characterization of Angiotensin II Receptors
Figure 1.10.3 The inhibition of [125 I]Ang II binding in intact Chinese hamster ovary cells expressing AT1A receptors. The IC50 values are 2.3 nM (Ang II), 22 nM (losartan), >1 µM (PD123177), 1.7 nM
4 月 10 日 丁香实验科研资讯① Cell开创性研究：图解单个细胞和单个胚胎的染色质结合蛋白摘要：来自美国匹兹堡大学生命科学学院，麻省大学医学院的研究人员利用一种开创性新方法：uliCUT＆RUN绘制了少量细胞（包括单细胞）和单个植入前胚胎的染色质上转录因子和DNA结合蛋白图谱。来自美国匹兹堡大学生命科学学院，麻省大学医学院的研究人员利用一种开创性新方法：uliCUT＆RUN绘制了少量细胞（包括单细胞）和单个植入前胚胎的染色质上转录因子和DNA结合蛋白图谱。这一研究成果公布在4月4日
(1992) The oligonucleotides we use are kits, contaning 20 decamers, from Operon Technologies Inc. . I. DNase I treatment of RNA mix and incubate for 30 min. at 37 °C : 50 µgRNA, 10 µl RNase inhibitor (1 U/µl), 1 µl
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