- ¥75
- ROBY
- 中国
- RY-WBST002a
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
1年
- 英文名：
Tris/Glycine Transfer Buffer for Western Blots and Native Gels，10X
- 供应商：
普京康利
- 规格：
500ml（Certified to 4C2T Quality System,Biological Reagent Grade）
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文献和实验SEMI-DRY ELECTROPHORETlC TRANSFER (WESTERN BLOTS)
that it is just as effective to use a slightly diluted version of the Tris/glycine running buffer used for SDS-polyacrylamide gels. Materials (for one transfer) Power supply capable of 12 - 100 V, 5 W 1 - 7 x 10 cm nitrocellulose sheet, 0.45 or 0.22 um pore
1. Run an SDS gel as usual. (It's nice to run a lane of prestained markers, which will transfer on to the blot and control for the transfer). 2. Transfer: make 4 liters of transfer buffer. Cut 3 pieces Whatman and one piece of nitrocellulose
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
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