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N-HA plasmid(with CMV promoter)
长期
北京百奥莱博科技有限公司
-20℃
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博主要从事生物试剂的生产和销*(代"售"),产品线涵盖YT480型N端HA标签融合蛋白质粒现货供应在内的分子生物学试剂产品,还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。
名称:YT480型N端HA标签融合蛋白质粒现货供应
产地:国产|进口
规格:1μg
英文名:N-HA plasmid(with CMV promoter)
品牌:百奥莱博
本质粒是用于在哺乳动物细胞中表达N端和HA tag(HA标签)融合的目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达;在多克隆位点的5"端含有一个可以编码HA标签的序列,因此可以表达出含有HA标签的融合蛋白,可以方便地使用抗HA的抗体来识别目的蛋白,有利于目的蛋白检测和分离纯化。质粒为卡那霉素抗性。转染细胞后,可使用G418筛选稳定表达目的蛋白的细胞株。
本质粒质粒的主要信息如下:
Feature Nucleotide | Position |
CMV promoter | 1-602 |
T3 promoter and T3 primer binding site | 620-639 |
HA tag | 679-705 |
multiple cloning site | 708-781 |
T7 promoter and T7 primer binding site | 824-845 |
SV40 polyA signal | 857-1240 |
f1 origin of ss-DNA replication | 1378-1684 |
bla promoter | 1709-1833 |
SV40 promoter | 1853-2191 |
neomycin/kanamycin resistance | ORF 2226-3017 |
HSV-thymidine kinase(TK)polyA signal | 3018-3476 |
pUC origin | 3605-4272 |
About this Antibody | |
Name | Cyclin D1 Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal residues of mouse Cyclin D1. |
Gene ID | 595 |
SwissProt | P24385 |
Synonyms | CCND1;BCL1; D11S287E; PRAD1; U21B31 |
Category | Cell Cycle / Checkpoint Control |
Background | Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. Cyclin D1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. Cyclin D1 has been found to be overexpressed in breast carcinoma. Its potential use as a biomarker was suggested . |
北京百莱博科技有限公司是克隆与表达产品的专业生产供应销*(代"售")商,恭候您的咨询选购YT480型N端HA标签融合蛋白质粒现货供应。
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