SimpleChIP® Universal qPCR Mas

CST官网该产品详情信息：http://www.cst-c.com.cn/products/88989.html。
该产品说明书：https://media.cst-c.com.cn/pdf/88989.pdf。

产品描述：

The SimpleChIP^® Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection.

This product is provided in 1 ml volumes sufficient for preparation of 100 qPCR reactions, and is compatible with both enzymatic and sonication-fragmented DNA samples from SimpleChIP^®enzymatic and sonication ChIP kits. This master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA, except primers and a DNA template.

产品使用信息：
Storage: This product is stable for 12 months when stored at -20°C and protected from light. It is stable for up to 30 freeze/thaw cycles and can be stored at 4°C, protected from light, for up to 1 month.

背景介绍：
Dye-based quantitative PCR (qPCR) uses real-time fluorescence of a double-stranded DNA (dsDNA) binding dye, most commonly SYBR^® Green I, to measure DNA amplification as it occurs during each cycle of PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle, or C_T value, can be determined. C_T values can be used to evaluate relative target abundance between two or more samples, or to calculate absolute target quantities in reference to an appropriate standard curve, derived from a series of known dilutions. qPCR is commonly used to detect and quantify target genes in genomic DNA that is enriched by chromatin immunoprecipitation (ChIP).
 

搭配高效 SimpleChIP® 通用 qPCR Master Mix，进行快速、灵敏、精确实时定量 PCR 分析

Q-PCR 定量分析：适合单个或少数已知结合位点的定量 PCR 分析; CST 提供经优化的 SimpleChIP® 通用 qPCR Master 预混液 #88989 ， 内含热启动 Taq DNA 聚合酶，独特校正染料，防止产物残留污染的 dUTP 及适用于直接肉眼观测的非荧光染料。包含除了模板和引物以外，用于 PCR 扩增和定量检测的所有组分。
 

与市场领先的 qPCR Mix 产品相比，SimpleChIP® 通用 qPCR Master Mix #88989 在灵敏度和线性方面都有明显提高。
 

如图: 经 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 处理 Hela 细胞，检测浓度稀释的系列 Input DNA 样品的扩增曲线。使用 SimpleChIP® Universal qPCR Master Mix #88989（左图）或市场领先 qPCR Mix(右图)，20ng (红色), 4 ng (橙色), 0.8 ng (紫色) 和 (蓝色) 的 input DNA 模板分别与SimpleChIP® Human α Satellite Repeat Primers #4486，进行定量 PCR检测。数据采用双重复样本检测。
 

与市场领先的 qPCR Mix 产品相比，SimpleChIP® 通用 qPCR Master Mix #88989 均获得优质定量 PCR 富集效率。

如图. 经 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) 和 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, TCF4/TCF7L2 (C48H11) Rabbit mAb #2569 和 Normal Rabbit IgG #2729 分别富集的 ChIP DNA，按照如图所示的引物进行检测，检测浓度稀释的系列 Input DNA 样品的扩增曲线，使用 SimpleChIP® 通用 qPCR Master Mix #88989 (蓝色/绿色柱)或市场领先 qPCR Mix (红色/紫色柱)。qPCR 定量结果为相对于 Input 的富集比值显示，其中 Input 设定值 为 1。

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https://www.cst-c.com.cn/about/orderInformation.jsp，进去后“查找您的分销商”选择您所在区域，即可看到联系方式。
 

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