The Quantus™ Fluorometer is a compact, dual-channel fluorometer designed to provide highly sensitive fluorescent detection when quantifying nucleic acids. The Quantus™ Fluorometer is optimized with preprogrammed settings for Promega QuantiFluor® Dye Systems (QuantiFluor® dsDNA, RNA and ssDNA Systems) to quantitate nucleic acids and offers the flexibility to create customized methods and quantitation settings for other fluorescent dyes.
Features
Easy, intuitive controls.
Calculates concentration automatically.
Store the last 50 readings and transfer data in real time.
Recommended for NGS and compatible with Illumina (HiSeq/miSeq), Roche (454) and LifeTech (ion Torrent and ion Proton) Systems.
Two fluorescent channels:
Red: Ex: 640nm shortpass (wavelengths up to 640nm); Em: 660–720nm
Blue: Ex: 495nm shortpass (wavelengths up to 495nm); Em: 510–580nm
Easily customize sample names, view concentration results, raw values, sample volumes and a quality checkCompact size at only 22.7 x 11.5 x 4Large 3-inch liquid crystal display screen
Intuitive navigation
Performance
Detection Sensitivity
Fluorescence-based nucleic acid quantification methods are more sensitive than absorbance-based methods, allowing you to quantitate low-level DNA samples accurately.
Custom protocols can be created for the Quantus™ Fluorometer to detect even lower amounts.
Broad Dynamic Range
Complement your absorbance-based methods and quantify your target molecule over a wider range of concentrations.
Quantitation of more dilute samples is possible using more input dsDNA per assay.
Calibration & Sample Measurement
The calibration process eliminates the need to generate a standard curve for each set of unknown samples. Simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements.
No computer is necessary to use the Quantus™ Fluorometer, but after installing the free Quantus™ Software you can automatically transfer your quantitation results from the Quantus™ Fluorometer to a personal computer in real time. Export results easily into Microsoft® Excel.
Design Features
Size
22.7 × 11.5 × 4.5cm (L × W × H)
Weight
400 grams
Linear Dynamic Range
Five orders of magnitude, assay-dependent
Detectors
Solid state silicon sensor
Excitation Filters
Red: 640nm shortpass (wavelengths up to 640nm) Blue: 495nm shortpass (wavelengths up to 495nm)
Emission Filters
Red: 660–720nm Blue: 510–580nm
Calibration Type
Single-point (blank and standard)
Sample Tube
We recommend Axygen thin-walled PCR tubes (Axygen Cat.# PCR-05-C, available from Fisher or VWR)
Automatic Sleep Mode
After 1 hour of inactivity
Power Requirements
5V, 0.2A Maximum
Operating System Requirements (for optional Quantus™ Software)
Windows® 8, Windows® 7 (32 or 64 bit) .NET Framework 4.0 or later At least one USB port available