
RNA pulldown试剂盒(RNA pulldown K
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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件:
4℃、-20℃
- 保质期:
1年
- 英文名:
RNA pulldown Kit
- 库存:
99999
- 供应商:
广州伯信生物科技有限公司
- CAS号:
暂无
- 规格:
40T
蛋白质与RNA的相互作用是许多细胞功能的核心,如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等,了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质(RBPs)。
实验原理:
针对目标区域设计脱硫生物素标记的特异性RNA探针,并与细胞蛋白提取物孵育。通过偶联磁珠,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。
产品优势:
1. 精准:高品质,稳定性好;
2. 易用:操作简单,容易上手;
3. 背景低:特异性高,背景少;
4. 自主品牌:拥有自主知识产权专利;
5. 快速省时:全部实验只需3小时
技术流程:
参考文献:
[1]Shen S,Wu Y,Chen J,et al.CircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene[J].Annals of the rheumatic diseases,2019,78(6):826-836.DOI:10.1136/annrheumdis-2018-214786
原文链接:https://ard.bmj.com/content/78/6/826
[2]Nan A,Jia Y,Li X,et al.Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis[J].Toxicological sciences : an official journal of the Society of Toxicology,2018,161(1):115-124.DOI:10.1093/toxsci/kfx203
原文链接:https://academic.oup.com/toxsci/article/161/1/115/4211135?searchresult=1
[3]Chen L,Nan A,Zhang N,et al.Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer[J].Molecular cancer,2019,18(1):13.DOI:10.1186/s12943-019-0943-0
原文链接:https://doi.org/10.1186/s12943-019-0943-0
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文献和实验- Gong Z, Zhu J, Chen J, Feng F, Zhang H, Zhang Z, Song C, Liang K, Yang S, Fan S, Fang X, Shen S. CircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications. Nat Commun. 2023 Aug 28;14(1):5242. IF=16.600.
- Yu Z, Lv Y, Su C, Lu W, Zhang R, Li J, Guo B, Yan H, Liu D, Yang Z, Mi H, Mo L, Guo Y, Feng W, Xu H, Peng W, Cheng J, Nan A, Mo Z. Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma. Cancer Res. 2023 Mar 2;83(5):700-719. IF=11.200.
- Dou R, Han L, Yang C, Fang Y, Zheng J, Liang C, Song J, Wei C, Huang G, Zhong P, Liu K, Peng Q, Peng C, Xiong B, Wang S. Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis. Clin Transl Med. 2023 Oct;13(10):e1432. IF=10.600.
- Li J, Li X, Zhou S, Wang Y, Ying T, Wang Q, Wu Y, Zhao F. Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration. Clin Transl Med. 2023 Sep;13(9):e1415. IF=10.600.
- Yang J, He W, Gu L, Zhu L, Liang T, Liang X, Zhong Q, Zhang R, Nan A, Su L. CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling. Transl Res. 2023 Jul;257:15-29. IF=10.170.
- Zheng Z, Wu M, Li H, Xu W, Yang M, Pan K, Ni Y, Jiang T, Zheng H, Jin X, Zhang Y, Ding L, Fu J. Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis. BMC Med. 2023 Apr 3;21(1):132. IF=9.300.
- Cao M, Ren Y, Li Y, Deng J, Su X, Tang Y, Yuan F, Deng H, Yang G, He Z, Liu B, Yao Z, Deng M. Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis. Int J Biol Sci. 2023 Jul 16;19(12):3678-3693. IF=9.200.
- Jia Y, Yan Q, Zheng Y, Li L, Zhang B, Chang Z, Wang Z, Tang H, Qin Y, Guan XY. Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer. J Exp Clin Cancer Res. 2022 Sep 29;41(1):287. IF=11.300.
- Shen L, Zhang J, Zheng Z, Yang F, Liu S, Wu Y, Chen Y, Xu T, Mao S, Yan Y, Li W, Zhang W, Yao X. PHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer. Int J Biol Sci. 2022 Aug 29;18(14):5459-5474. IF=9.200.
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
genomic regions in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
♦ 适用范围:适用于快速提取植物组织细胞总RNA本试剂盒在室温储存12个月不影响使用效果。♦ 储存事项:1. 所有的溶液应该是澄清的,如果环境温度低时溶液可能形成沉淀,此时不应该直接使用,可在37℃水浴加热几分钟,即可恢复澄清。2. 不合适的储存于低温(4℃或者-20℃)会造成溶液沉淀,影响使用效果,因此运输和储存均在室温下(15℃-25℃)进行。3. 避免试剂长时间暴露于空气中产生挥发、氧化、PH值变化,各溶液使用后应及时盖紧盖子。♦ 产品介绍:独特的裂解液/β-巯基乙醇迅速裂解细胞和灭










