产品介绍: 染色质免疫沉淀 (ChIP) 是研究体内蛋白质与 DNA 相互作用的理想技术,是目前确定与特定蛋白结合的基因组区域,或确定与特定基因组区域结合的蛋白质的最好方法。ChIP 与其他实验技术相结合,可用于不同用途的检测。ChIP-Seq 方法是对特异性地富集目的蛋白结合的 DNA 片段进行纯化与文库构建,然后对富集得到的 DNA 片段进行高通量测序。
实验原理: 在活细胞状态下把细胞内的蛋白质和 DNA 交联,超声波将其随机切断为一定长度范围内的染色质小片段,然后用所研究的目的蛋白质特异性抗体免疫沉淀蛋白质-DNA 复合体,从而特异性地富集目的蛋白结合的 DNA 片段。
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